Experimental animal study on alcoholic hepatitis treated by Ruangan Sanjie Decoction
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HAN Bo1 QING Xian-chun1▲ JI Jun-xia2 ZHANG Zhi-gang2 QING Xiao-xing3 LI Rong4 LIU Jing1 QING Xiao-qi5
1.Shanxi University of Chinese Medicine,Jinzhong 030619,China;2.Taiyuan Infectious Disease Hospital,Sanxi Province,Taiyuan 030012,China;3.Institute of Medical Microbiology,University of Giessen,Giessen City,Hessen State D-35392,Germany;4.Tianjin Jiexi Cancer Hospital,Tianjin 300384,China;5.Beijing University of Chinese Medicine,Beijing 100029,China
Abstract：Objective To study the effect of Ruangan Sanjie Decoction on alcoholic hepatitis by animal experiment.Methods Forty-eight male Wistar rats were selected and randomly divided into control group,model group,low-dose group and high-dose group according to statistical principles,with 12 in each group.The ratio of weight of Chinese herbal medicines to rat body weight in the low-dose group was 10 g to 1 kg,while in the high-dose group,the ratio was 20 g to 1 kg.In the control group,equal volume of fodder and distilled water was supplied,but without traditional Chinese herbal medicines for gastric irrigation.Fodder was provided in both the model group and the control group.For the other two groups,besides normal feeding,traditional Chinese decoction instead of distilled water in the same volume was used for gastric irrigation.After 42 d modeling,specimens were collected and the body weight,liver wet weight,liver index,and liver bio-chemical indices related to liver function were measured,including liver weight,liver index,rat body weight,alanine aminotransferase(ALT),aspartate aminotransferase(AST),AST/ALT,albumin(Alb),total protein(TP),ethanol dehydrogenase(ADH),total bilirubin(TBil),methylene dioxyamphetamine(MDA),superoxide dismutase(SOD),and pathological tissue.Results The liver weight and liver index of the model group were higher than those of the control group,the weight of the rats was lower than that of the control group,the liver weight of the low-dose group was heavier than that of the control group (P＜0.01).The liver weight and liver index of the high-dose group were lower and the body weight was heavier compared with those in the model group,the liver index of the low-dose group was lower than that of the model group,and the body weight of the rats was heavier compared with those in the model group (P＜0.01).The liver weight of the high-dose group was lower than that of the low-dose group(P＜0.01).The AST/ALT,ALT,and AST in the model group and the low-dose group were higher than those in the control group (P＜0.01).The AST/ALT,ALT,and AST in the high-dose group were lower than those in the model group and the low-dose group (P＜0.01).Alb and TP were lower in the model group and the low-dose group than in the control group (P＜0.01).The TBil was higher in the model group than in the control group (P＜0.01).The Alb and TP were lower in the high-dose group than in the model group and the low-dose group (P＜0.01).The TBil level in the low-and high-dose groups was higher than that in the model group (P＜0.01).The levels of MDA in the model group and the low-dose group were higher than those in the control group,and the SOD levels were lower than those in the control group(P＜0.01).The MDA level in the high-dose group was lower than that in the low-dose group,the SOD level in the high-dose group was higher than that in the low-dose group and model group(P＜0.01).The values of steatosis and inflammatory infiltration in the model group,the low-dose group,and the high-dose group were all higher compared with those in the control group (P＜0.01).The steatosis and inflammatory infiltration values in the high-dose group were lower than those in the model group and the low-dose group(P＜0.01).Conclusion Ruangan Sanjie Decoction has a certain therapeutic effect on alcoholic hepatitis.
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