肠系膜静脉血栓大鼠肠道组织病理学变化的实验研究

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摘要目的观察不同范围肠系膜静脉血栓大鼠肠道组织病理学变化。　方法按随机原则将80只SD大鼠分为A、B两组，每组40只。A组：结扎肠道内径8 cm范围肠系膜静脉一级分支及边缘静脉；B组：结扎肠道内径6 cm范围肠系膜静脉一级分支及边缘静脉。观察两组大鼠一般状态、腹腔大体情况；切取肠道组织标本，HE染色，观察两组不同时间点肠道组织病理学变化。　结果 A组：术后3 h肠道组织淤血水肿；术后8 h出现血性腹水，并出现便血，部分区域黏膜组织坏死脱落；术后24 h部分区域平滑肌组织坏死；术后48 h上述改变加重；术后72 h肠道透壁性梗死。B组：术后3 h肠道组织淤血水肿；术后8 h出现便血，部分区域黏膜组织脱落坏死；术后24 h出现血性腹水，部分区域肠道平滑肌坏死；术后48 h上述改变未进行性加重；术后72 h肠道损害恢复。　结论肠系膜静脉血栓波及的范围越大，发生肠坏死的概率越高；血性腹水不能作为肠系膜静脉血栓剖腹探查的手术指征；血便是肠系膜静脉血栓减轻肠道淤血的方式；保持肠道平滑肌连续性的完整对肠系膜静脉血栓的预后非常关键。

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	陈建立
	陈俊卯
	王长友
	张国志
	张瑞兴
	崔明新
	曹文斌

关键词 ：肠系膜静脉血栓, 肠道损害, 平滑肌坏死, 动物实验

Abstract：Objective To observe the pathological changes of intestinal tissue in rats with different ranges of mesenteric venous thrombosis.Methods Eighty rats were evenly divided into two groups in random with 40 rats in each group.In group A，inner diameter of intestinal tract at 8 cm in first grade branch of superior mesenteric vein and marginal vein was ligated.In group B，inner diameter at 6 cm was closed.The general condition and condition of abdominal cavity were observed.The pathological changes of intestinal tissue at different time points between the two groups were observed after fetching intestinal tissue sample and HE staining.Results In group A，congestion and edema of intestinal tissue were occurred 3 hours after surgery，and hemorrhagic ascites with hematochezia 8 hours after surgery together with necrosis and falling of mucous tissue in partial region.Necrosis of smooth muscle tissue was developed 24 hours after surgery in partial region，and was worsened 48 hours after surgery.72 hours after surgery，transmural infarct of intestine was presented.In group B，there was congestion and edema of intestinal tissue 3 hours after surgery，and hematochezia and necrosis and falling of mucous tissue in partial region 8 hours after surgery.24 hours after surgery，hemorrhagic ascites was occurred together with necrosis of smooth muscle tissue in partial region.48 hours after surgery，no deterioration was detected on these above mentioned symptoms.The intestinal damage was recovered 72 hours after surgery.Conclusion The wider the range of mesenteric venous thrombosis is，the higher the incidence of intestinal necrosis becomes.Hemorrhagic ascites cannot be considered as a surgical indicator on abdominal laparotomy of mesenteric venous thrombosis. Bloody defecation is an approach to alleviate intestinal congestion due to mesenteric venous thrombosis.Maintenance of completion on intestinal smooth muscle succession plays a key role in prognosis of mesenteric venous thrombosis.

Key words： Mesenteric venous thrombosis Intestinal damage Smooth muscle necrosis Animal experiment

引用本文:

陈建立;陈俊卯;王长友;张国志;张瑞兴;崔明新;曹文斌. 肠系膜静脉血栓大鼠肠道组织病理学变化的实验研究[J]. 中国当代医药, 2016, 23(10): 8-12.
CHEN Jian-li;CHEN Jun-mao;WANG Chang-you;ZHANG Guo-zhi;ZHANG Rui-xing;CUI Ming-xin;CAO Wen-bin. Experimental study of pathological changes on intestinal tissue in mesenteric venous thrombosis rats. 中国当代医药, 2016, 23(10): 8-12.

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［1］	陈建立，张国志，常艳.肠系膜静脉血31例［J］.世界华人消化杂志，2012，20（2）：167-170.
［2］	陈建立，张国志，陈俊卯.影响肠系膜静脉血栓预后的相关原因分析［J］.中国全科医学，2012，15（4c）：1389-1390.
［3］	陈建立，张国志，常艳.急性肠系膜静脉血栓临床特点及预后影响因素分析［J］.中国全科医学，2012，15（3c）：1043-1044.
［4］	Acosta S，Krantz P.Trends in prevalence of fatal surgical diseases at forensic autopsy［J］.ANZ J Surg，2007，7（8）：718-721.
［5］	HortonKM，Fishman EK.Multidetector CT angiography in the diagnosis of mesenteric ischemia［J］.Radiol Clin North Am，2007，45（2）：275-288.
［6］	Levy AD.Mesenteric ischemia［J］.Radiol Clin North Am，2007，45（4）：593-599.
［7］	Ko lbel T，Lindh M，Holst J，et al.Extensive acute deep vein thrombosis of the iliocaval segment：midterm results of thrombolysis and stent placement［J］.J Vasc Interv Radiol，2007，18（2）：243-250.
［8］	Svensson PJ，Dahlba ck B.Resistance to activated protein Cas a basis for venous thrombosis［J］.N Engl J Med，1994，330 （8）：517-522.
［9］	宋明，王宇.肠系膜静脉血栓形成进展［J］.国外医学外科学分册，2001，28（5）：280-283.
［10］	张健，王玉波，张强.急性肠系膜静脉血栓形成的非手术治疗［J］.中国实用外科杂志，2003，23（4）：220-222.
［11］	Hedayati N，Riha GM，Kougias P，et al.Prognostic factors and treatment outcome in mesenteric vein thrombosis［J］. Vasc Endovascular Surg，2008，42（3）：217-224.
［12］	Hotoleanu C，Andercou O，Andercou A.Mesenteric venous thrombosis：clinical and therapeutical approach［J］.Int Angiol，2008，27（2）：462-465.
［13］	Gore RM，Yaghmai V，Thakrar KH，et al.Imaging in intestinal ischemic disorders［J］.Radiol Clin North Am，2008，46 （10）：845-875.
［14］	Brandt LJ，Boley SJ.AGA technical review on intestinal ischemia［J］.Gastroenterology，2000，118（5）：954-968.
［15］	Berry FB，Bougas JA.Agnogenic venous mesenteric thrombosis［J］.Ann Surg，1950，132（3）：450-474.
［16］	North JP，Wolleman OJ.Venous mesenteric occlusion in the course of migrating thrombophlebitis［J］.Surg Gynecol Obstet，1952，95（6）：665-671.
［17］	EgebergO.Inheritedantithrombindeficiencycausingthrombophilia［J］.Thromb Diath Haemorrh，1965，13（5）：516-530.
［18］	Cohn DM，Roshani S，Middeldorp S.Thrombophilia and venous thromboembolism：implications for testing［J］.Semin Thromb Hemost，2007，33（6）：573-581.
［19］	AmitranoL，BrancaccioV，GuardascioneMA，etal.Highprevalence of thrombophilic genotypes in patients with acute mesenteric vein thrombosis［J］.Am J Gastroenterol，2001，96（1）：146-149.
［20］	Janssen HL，Meinardi JR，Vleggaar FP，et al.Factor V Leiden mutation，prothrombin gene mutation，and deficiencies in coagulation inhibitors associated with Budd-Chiari syndrome and portal vein thrombosis：results of a case control study［J］.Blood，2000，96（12）：2364-2368.