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| Effect and mechanism of preoperative Emodin combined with Cisplatin chemotherapy on WIG-1 gene expression in human esophageal squamous cell carcinoma |
| XIAO Ren-bin PENG Hui WANG Jiang-tao WEN Zhao-ming ZHOU Kai-liang WU Juan▲ |
| Department of Oncology, Xiangyaping Mine Cooperative Hospital, Jiangxi Province, Pingxiang 337000, China |
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Abstract Objective To investigate the effect and mechanism of preoperative Emodin combined with Cisplatin on the expression of wild-type p53-induced gene 1 (WIG-1) in human esophageal squamous cell carcinoma. Methods Forty patients with esophageal squamous cell carcinoma admitted to our hospital from March 2017 to October 2018 were selected as subjects, all patients were diagnosed by preoperative pathological examination. Patients were given Emodin combined with Cisplatin for preoperative chemotherapy after diagnosis. After three consecutive courses of treatment,patients were operated on, according to the different tissues taken during the operation, patients were divided into observation group and control group, with 20 cases in each group. In the observation group, the lesion tissue was taken during the operation, while in the control group, the adjacent tissue was taken during the operation (distance from the tumor site (≥3 cm). The expression of WIG-1 in tissues of the two groups and the expression of WIG-1 before and after operation in the observation group were measured by reverse transcription-polymerase chain reaction (RT-PCR).Results The expression level of WIG-1 in the observation group was 0.68±0.11, higher than 0.04±0.01 in the control group, the difference was statistically significant (P＜0.05). The expression level of WIG-1 in esophageal squamous cell carcinoma tissues after operation in the observation group was 0.25±0.05, lower than 0.68±0.11 before operation, the difference was statistically significant (P＜0.05). Conclusion The expression level of WIG-1 in normal tissues is low.Preoperative Emodin combined with Cisplatin chemotherapy is helpful to reduce the expression level of WIG-1 in esophageal squamous cell carcinoma patients, improve the prognosis of patients, and provide molecular basis for the treatment of esophageal squamous cell carcinoma.
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