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| Influence of vitamin D on the mesenchymal transformation of alveolarⅡepithelial cells induced by TGF-β1in rats |
| QI Yun-feng1 HUO Ru-jie1 LIU Dai1 CHAO Wei-guan2 TIAN Xin-rui1▲ |
1.Department of Respiratory Medicine,the Second Hospital of Shanxi Medical University,Shanxi Province,Taiyuan 030001,China;
2.Department of the Science of Nuclear Medicine,the First Hospital of Shanxi Medical University,Shanxi Province,Taiyuan 030001,China |
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Abstract Objective To investigate the influence of vitamin D (VD)on the mesenchymal transformation of alveolarⅡepithelial cells(RLE-6TN)induced by TGF-β1in rats.Methods RLE-6TN cells were cultured in vitro,and were divided into the control group(group A),the TGF-β1group(group B),the TGF-β1＋VD group(group C),the TGF-β1＋ICG001 group(group D),the TGF-β1＋VD＋ICG001 group(group E).The cells were treated with VD and/or VD＋ICG001 at first,and TGF-β1was added into the culture solution after 24 h,the Western blot was used to detected the α-SMA,Fibronectin (Fn)and E-cadherin protein levels after 48 h.Results Under the microscope,in group A,cells adhered to the wall and showed a paving stone with tight junctions.The gap in group B was significantly increased and the spindle was obviously deformed.The cells in groups C and group D showed more paving stone-like changes than those in group B,and the cells gap decreased.Most of the cells in the group E were mostly paved stone-like changes,the gap was smallest and morphology was close to the normal group.Western blot analysis showed that the expression levels of α-SMA and Fn in groupB,C,D,E were higher than that in group A,but the expression of E-cadherin was lower than that in group A,the differences were statistically significant(P＜0.05).The levels of α-SMA and Fn in group C,D,E were lower than that in group B,but the expression of E-cadherin was higher than that in group B,the differences were statistically significant(P＜0.05).The expression levels of α-SMA and Fn in group C and group D were higher than that in group E,but the expression of E-cadherin was lower than that in group E,and the differences were statistically significant(P＜0.05).There were no significant differences in the expression levels of α-SMA,Fn and E-cadherin between group C and group D (P＞0.05).Conclusion Vitamin D can inhibited the mesenchymal transition of alveolarⅡ epithelial cells induced by TGF-β1in rats.
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| [1] |
Moustakas A，Heldin CH.Mechanisms of TGFβ -induced epithelial-mesenchymal transition[J].J Clin Med，2016，5（7）：63-70.
|
| [2] |
Fang L，Wu J，Huang T，et al.TGF-β1stimulates epithelialmesenchymal transition mediated by ADAM33[J].Exp Ther Med，2018，15（1）：985.
|
| [3] |
Tian XR，Zhang J，Tan TK，et al.Association of beta-catenin with P-Smad 3 but not LEF-1 differentiates in vitro profibrotic and anti-inflammatory effects of TGF-beta 1[J].Cell Sci，2012，126（1）：67-76.
|
| [4] |
Kim SH，Pei QM，Jiang P，et al.Effect of active vitamin D3on VEGF-induced ADAM33 expression and proliferation in human airway smooth muscle cells：implications for asthma treatment[J].Resp Res，2017，18（1）：7-13.
|
| [5] |
Berraies A，Hamzaoui K，Hamzaoui A.Link between vitamin D and airway remodeling[J].J Asthma Allergy，2014，2014（default）：23-30.
|
| [6] |
Hansdottir S，Monick MM，Hinde SL，et al.Respiratory epithelial cells convert inactive vitamin D to its active form：potential effects on host defense[J].J Immunol，2008，181（10）：7090-7095.
|
| [7] |
Su MH，Garcia LA，Contreras JR，et al.1，25-Vitamin-D3Promotes cardiac differentiation through modulation of the Wnt signaling pathway[J].J Molendo，2014，53（3）：303-317.
|
| [8] |
Ramirez AM，Wongtrakool C，Welch T，et al.Vitamin D inhibition of pro-fibrotic effects of transforming growth factor beta1inlungfibroblastsandepithelialcells[J].JSteroidBiochem，2010，118（3）：142-150.
|
| [9] |
Henderson WR，Chi EY，Ye X，et al.Inhibition of Wnt/βcatenin/CREB binding protein （CBP） signaling reverses pulmonary fibrosis[J].Proc Natl Acad Sci U S A，2010，107（32）：14309-14317.
|
| [10] |
Fehrenbach H，Wagner C，Wegmann M.Airway remodeling in asthma：what really matters[J].Cell Tissue Res，2017，367（3）：1-19.
|
| [11] |
Yang L，Jiao X，Wu J，et al.Cordycepssinensis inhibits airway remodeling in rats with chronic obstructive pulmonary disease[J].Exp Ther Med，2018，15（3）：2731.
|
| [12] |
Li LH，Lu B，Wu HK，et al.Apigenin inhibits TGF-β1-induced proliferation and migration of airway smooth muscle cells[J].Int J Clin Exp Pathol，2015，8（10）：12557.
|
| [13] |
Zheng G，Lyons JG，Tan TK，et al.Disruption of E-cadherin by matrix metalloproteinase directly mediates epithelialmesenchymal transition downstream of transforming growth factor-beta1 in renal tubular epithelial cells[J].Am J Pathol，2009，175（2）：580-591.
|
| [14] |
Guo Y，Xiao L，Sun L，et al.Wnt/beta-catenin signaling：a promising new target for fibrosis diseases[J].Physiol Res，2012，61（4）：337-343.
|
| [15] |
Koopmans T，Crutzen S，Menzen MH，et al.Selective targeting of CREB-binding protein/β-catenin inhibits growth of and extracellular matrix remodelling by airway smooth muscle[J].Br J Pharmacol，2016，173（23）：3327-3341.
|
| [16] |
Korn S，Hübner M，Jung M，et al.Severe and uncontrolled adult asthma is associated with vitamin D insufficiency and deficiency[J].Resp Res，2013，14（1）：25-32.
|
| [17] |
Niruban SJ，Alagiakrishnan K，Beach J，et al.Association between vitamin D and respiratory outcomes in Canadian adolescents and adults[J].J Asthma，2015，52（7）：653-661.
|
| [18] |
Somashekar AR，Prithvi AB，Gowda MN.Vitamin D levels in children with bronchial asthma[J].J Clin Diagn Res，2014，8（10）：PC04-PC07.
|
|
|
|
