大鼠脑出血后AQP-4、CRP及VEGF的表达与脑水肿的相关性研究

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Abstract Objective To make discussions over the expressions at different times AQP_4，CRP and VEGF after cerebra1 hemorrhage of rat as we11 as the re1evance with cerebra1 edema.Methods 90 rats were divided into two groups∶contro1 group　（inc1uding 18 rats）and experimenta1 group　（inc1uding 72 rats）.The contro1 group was the mode1 of fa1se b1eeding.As to the experimenta1 group，the stereo_tactic techniques were adopted to estab1ish the mode1 of cerebra1 hemorrhage of rats.After the cerebra1 hemorrhage，observations were made on the changes of rats′neuro1ogica1 functions at different time.After that，the heads of rats in the two groups were cut to get the brain.The ELISA method was adopted to test the serum CRP and VEGF，and the immunohisto_chemica1 method was adopted to test the brain tissue AQP_4. Results The foot imprinting test from grade 6 h to 14 d in the experimenta1 group were higher than those in the contro1 group（P＜0.05 or P＜0.01）.AQP_4，CRP and VEGF expression increased at 6 h，and increased obvious1y at 24 h，48 h，3 d and 7 d.Besides，the differences were significant（P＜0.01）.Conclusion After the cerebra1 hemorrhage，there is over_expression of AQP_4，CRP，and VEGF；besides，the dynamic changes are positive1y corre1ative with the neuro1ogica1 function reaction and cerebro_ce11u1ar edema.It is specu1ated that the above three factors have participated in the formation and regression of cerebra1 hemorrhage and edema.

Key words： Incerebra1 hemorrhage Aquaporin_4 C_reactive protein Vascu1ar endothe1ia1 growth factor Brain edema

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	ZHANG Bo
	LIU Jian

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ZHANG Bo,LIU Jian. Correlation between AQP_4，CRP，VEGF expression and brain edema after cerebral hemorrhage in rats[J]. 中国当代医药, 2016, 23(12): 125-128.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I12/125

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