Analysis of serum metabolism of qi and yin deficiency syndrome in the compensated stage of chronic renal failure based on 1H-NMR technology
ZHANG Min-ji1 GAO Ji-ning2▲ ZHANG Xue-qi3
1. Department of Traditional Chinese Medicine, Jincheng Rehabilitation Hospital, Shanxi Province, Jincheng 048000,China;
2. The Third Department of Nephrology, Shanxi Hospital of Integrated Traditional and Western Medicine, Shanxi Province, Taiyuan 030013, China;
3. Department of Anesthesiology, Sihe Hospital, Jincheng Grand Hospital, Shanxi Province, Jincheng 048015, China
Abstract：Objective To analyze the serum metabolism of qi and yin deficiency syndrome in the compensated stage of chronic renal failure based on 1H-NMR technology. Methods From January to November 2019, 15 voluntary patients with qi and yin deficiency syndrome in the compensated stage of chronic renal failure admitted to the Third Department of Nephrology, Shanxi Hospital of Integrated Traditional and Western Medicine were selected as the disease group, and 15 healthy patients′ family members and volunteers were selected as the control group. Fasting venous blood was collected and prepared for sample processing. 1H-NMR technology combined with principal component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA) and other diversified analytical methods were used to find endogenous compounds and compare metabolite differences between groups. Results In the disease group, the contents of seven metabolic differences including arginine, proline and lipid were higher than those in the control group,and the contents of two kinds of choline and phosphatidylcholine were lower than those in the control group, the differences were statistically significant (P＜0.05). Conclusion It is initially found that there is a specific metabolic profile in the syndrome of deficiency of both qi and yin in the compensatory phase of chronic renal failure. The metabolic disorder of qi and yin deficiency syndrome in the compensatory phase of CRF may mainly involve amino acid and lipid metabolism. Metabonomics technology can provide a more comprehensive reference for the modern research and clinical practice of traditional Chinese medicine kidney disease,and help realize the ultra-early diagnosis and treatment of chronic renal failure.
张敏继；高继宁；张学奇. 基于1H-NMR技术的慢性肾衰竭代偿期气阴两虚证血清代谢分析[J]. 中国当代医药, 2021, 28(19): 8-11.
ZHANG Min-ji1 GAO Ji-ning2▲ ZHANG Xue-qi3. Analysis of serum metabolism of qi and yin deficiency syndrome in the compensated stage of chronic renal failure based on 1H-NMR technology. 中国当代医药, 2021, 28(19): 8-11.
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