大蒜有效成分组方的药效学实验研究

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摘要目的观察大蒜有效成分组方（Y 为大蒜油、Z 为大蒜皂苷、D 为大蒜多糖）对接种S180 小鼠生命延长率和抗炎作用的疗效。方法实验1：昆明小鼠，雄性，80 只，按照随机数字表法分为模型组，溶剂组，环磷酰胺组，大蒜油组，受试药物（Y+Z+D）高、次高、中、低剂量，共8 组，每组各10 只。各组给予相应药物10 d 后开始观察并记录各组小鼠的存活时间、生命延长率。实验2：昆明小鼠72 只，雌雄各半，将小鼠按照随机数字表法分为6 组，即模型组，阿司匹林组，大蒜YZD 高、次高、中、低剂量组（每组各12 只，雌雄各半），以上各组给予相应的药物，每天1 次，连续给药7 d。末次给药2 h 后将小鼠处死，剪下两耳称重，计算肿胀度和肿胀抑制率。结果实验1 结果显示，（Y+Z+D）高剂量组和大蒜油组的动物存活天数与模型组比较，差异有统计学意义（P＜0.05）；（Y+Z+D）高剂量组和大蒜油组的动物存活天数长于其他各组，差异有统计学意义（P＜0.05）；（Y+Z+D）高剂量组和大蒜油组的生命延长率亦高于其他各组。实验2 结果显示，大蒜YZD 高剂量具有一定的抗炎的作用。结论大蒜有效成分组方（Y+Z+D）高剂量具有一定的抗肿瘤和抗炎作用。

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关键词 ：大蒜有效成分, 大蒜油, 大蒜多糖, 大蒜皂苷, 药效学, 实验研究

Abstract：Objective To observe the efficacy of the effective part formula of garlic (Y means garlic oil, Z means garlic saponin,D means garlic polysaccharide)on the life extension rate and anti-inflammatory of inoculated S180 mice. Methods In experiment 1, 80 male Kunming mice were divided into model group, solvent group, Cyclophosphamide group,garlic oil group, the test effective components high dose (Y+Z+D) group, secondary high dose (Y+Z+D) group, medium dose (Y+Z+D) group and low dose (Y+Z+D) group according to the random number table method, with 10 mice in each group. The survival time and life extension rate of each mouse in each group were observed and recorded after 10 days of administration of the corresponding drugs. In experiment 2, 72 Kunming mice, including 36 male mice and 36 female mice, were divided into model group, Aspirin group, high dose garlic YZD group, secondary high dose garlic YZD group, medium dose garlic YZD group and low dose garlic YZD group, with 12 mice in each group (half male and half male). The mice of above groups were given corresponding test drugs once a day for 7 days. Two hours after the last administration, the mice were killed. Each ear was cut and weighed, and the swelling degree and swelling inhibition rate were calculated. Results The results of experiment 1 showed that the survival time of animals in the high dose (Y+Z+D)group and garlic oil group was statistically significant compared with the model group (P＜0.05). The survival time of animals in the high dose (Y+Z+D) group and garlic oil group was longer than that in the other groups, and the difference was statistically significant (P＜0.05). The life extension rate of the high dose (Y+Z+D) group and the garlic oil group was also higher than that of the other groups. The results of experiment 2 showed that high dose garlic YZD has a certain anti-inflammatory effect. Conclusion The effective part formula of garlic (high dose of Y+Z+D) has certain anti-tumor and anti-inflammatory effect.

Key words： Effective parts of garlic Garlic oil Garlic polysaccharide Garlic saponin Pharmacodynamics Experimental study

收稿日期: 2020-03-17

基金资助:国家科技重大专项项目（2009ZX09301-005-11）；
国家自然科学基金青年科学基金项目（81603714）

作者简介: 那坤（1980-），男，黑龙江人，博士，副主任医师，从事中西医结合诊治内科疾病的临床研究工作 ▲通讯作者：胡华刚（1983-），男，山东人，博士，副研究员，从事中西医结合慢性病康复基础研究

引用本文:

那坤；张立石；薛欣；王瑞海；柏冬；胡华刚. 大蒜有效成分组方的药效学实验研究[J]. 中国当代医药, 2021, 28(12): 4-007.
NA Kun；ZHANG Li-shi； XUE Xin； WANG Rui-hai； BAI Dong； HU Hua-gang. Research on the correlation between serum level of NT-proBNP and cardiac function and ventricular remodeling in elderly patients with chronic congestive heart failure. 中国当代医药, 2021, 28(12): 4-007.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2021/V28/I12/4

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