Distribution of methylenetetrahydrofolate reductase and methionine synthase reductase gene polymorphisms in women of child-bearing age at Lichuan City,Hubei Province
XU Min1 JIANG Hong2▲ MOU Dan1 MOU Yao-hua1 LAN Qiao1 HE Ling-yu1 QIN Lu1 LIU Xin-yu3
1.Department of Obstetrics,Lichuan People′s Hospital,Hubei Province,Lichuan 445400,China;
2.Clinical Laboratory,Huangshi Maternal and Child Health Care Hospital,Hubei Province,Huangshi 435000,China;
3.Clinical Laboratory,the 324thHospital of PLA,Chongqing 400000,China
Abstract：Objective To compare the C667T and A1298C loci of methylenetetrahydrofolate reductase (MTHFR)and the methionine synthetase reductase(MTRR)A66G locus among Tujia and Han women of reproductive age in the People′s Hospital of Lichuan City,Enshi Prefecture,Hubei Province.For the case of point polymorphism distribution,the hereditary characteristics of the locus were studied.Methods A total of 681 cases of Han and 553 cases of Tujia fertile women were selected as subjects,from those building cards at Lichuan People′s Hospital of Hubei Province from January,2015 to December 2017.Oral epithelial cells were collected to extract genome DNA,Taqman-MGB technology was used to detect MTHFR and MTRR gene polymorphisms,and then compared with those in other nationalities in China.Results The gene frequency of MTHFR C677T locus of Tujia women in Lichuan People′s Hospital of Enshi Prefecture,Hubei Province was compared with that of Yanbian Korean,Nanning Zhuang,Ursukazak and Yinchuan Hui women,the differences were statistically significant(P<0.05).The gene frequency at the MTHFR A1298C site was statistically significant compared with that of the Nanning Zhuang and Ursukazak women,the differences were statistically significant(P<0.05).The gene frequency at the MTRR A66G site was compared with that of Ursukazak and Xinjiang Uygur women,and the differences were statistically significant(P<0.05).There was a complete linkage disequilibrium between the two points of MTHFR C677T and A1298C of Tujia in Lichuan City (D′=1.0,r2=0.508).There was a complete linkage disequilibrium between two sites of MTHFR C677T and A1298C of Han nationality in Lichuan City (D′=1.0,r2=0.424).Conclusion There is no significant difference in the polymorphism distribution of MTHFR and MTRR between Tujia and Han populations in Lichuan City,Enshi Prefecture,Hubei Province.However,the above gene distribution in women in the region is region-specific and different from other regions.
许敏； 江鸿； 牟丹； 牟耀华； 蓝巧； 何铃钰； 覃 露； 刘欣雨. 湖北省利川市育龄妇女亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性分布研究[J]. 中国当代医药, 2019, 26(7): 192-196.
XU Min JIANG Hong； MOU Dan； MOU Yao-hua； LAN Qiao； HE Ling-yu； QIN Lu； LIU Xin-yu. Distribution of methylenetetrahydrofolate reductase and methionine synthase reductase gene polymorphisms in women of child-bearing age at Lichuan City,Hubei Province. 中国当代医药, 2019, 26(7): 192-196.
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