Technical essentials for molecular biological method on identification of Fritillaria cirrhosa and its decoction pieces
YAN Hai-xia TAN Cheng DU Xiao-wei CHEN You-gen FU Xin-tong▲
Department of Traditional Chinese Materials,Beijing Institute for Drug Control（Beijing Center for Health Food and Cosmetic Control）,Beijing 102206,China
Abstract：TraditionalChinese Medicine is traditional medicine of china,which has extensive use in clinic treatment and daily health-care.The quality of Chinese medicinal materials and Traditional Chinese Medicine decoction pieces relates greatly to the clinical curative effect and security,which is also very important to the development of Traditional Chinese Medicine property.The identification of Chinese medicinal materials and Chinese medicinal decoction pieces is a focal point in traditional Chinese medicines profession,with the development of life science and technology leap,a variety of molecular biology technology is gradually used in medicinal plants and animals and the base source identification of Chinese Medicinal Materials,achieved good results.The"Pharmacopoeia of the People′s Republic of China" since 2010,contains the Chinese medicinal materials and medicinal slices(e.g,Agkistrodon,wu pin snakes and Fritillaria cirrhosa)molecular biology method,is to adopt new technology,new method to control the quality of traditional Chinese medicine.Standard has been four years,but brother units generally reflect the various technical problems encountered in the inspection process,it can′t solve.Identification based on the molecular biology of Fritillaria cirrhosa,for example,by summing up the main points that need to pay attention to the process of technology and common problems,aiming at will or are engaged in the inspection work colleagues to provide experience for reference,so that more efficient to complete the experiment successfully.
闫海霞 ；谭成； 杜小伟； 陈有根 ；傅欣彤. 分子生物学方法鉴别川贝母及饮片的技术要点探析[J]. 中国当代医药, 2017, 24(6): 92-95.
YAN Hai-xia ；TAN Cheng； DU Xiao-wei；CHEN You-gen； FU Xin-tong. Technical essentials for molecular biological method on identification of Fritillaria cirrhosa and its decoction pieces. 中国当代医药, 2017, 24(6): 92-95.
Pathak H，Jaroli DP.DNA finger printing analysis of enght species of Dendrobium found in western ghats using RAPD and ISSR[J].Indian J Funda App Life Sci，2012，1（2）：306-309.
Kang JY，Lu JJ，Qiu S，et al.Dendrobium SSR markers play a good role in genetic diversity and phylogenetic analysis of Orchidaceae species[J].Sci Hortic，2015，183（183）：160-166.
Yao H，Song J，Liu C，et al.Use of ITS2 region as the uninversal DNA barcode for plants and animals[J].PLoS One，2010，5（10）：e13102．
[17]
Li DZ，Gao LM.Comparative analysis of a large dataset indicates thatinternal transcribed spacer（ITS）should be incorporatedinto the core barcode for seed plants[A]//Proceedings of the National Academy of Sciences of the United States of America[C].2011，108（49）：19641-19646．
京公网安备 11010502046607号