Impact of Valproic Acid on drug resistance of HL-60 cell
HU Jian-feng1 YIN Song-mei2▲ XIE Shuang-feng2 NIE Da-nian2 LI Yi-qing2
1.Department of Oncology,Shunde District Hospital of Traditional Chinese Medicine in Foshan City of Guangdong Province,Foshan 528333,China;
2.Department of Blood,Sun Yat-sen Memorial Hospital of Zhongshan University in Guangdong Province,Guangzhou 510120,China
Abstract：ObjectiveTo explore the impact of Valproic Acid(VPA)on drug resistance of HL-60 cells（HL-60/HT）resistant harringtonine.MethodsHL-60/HT cell strain was established by incremental pressure culture and divided into 3 groups:Ara-C group,VPA group and VPA+Ara-C group.MTT method was used to determine the change of proliferation of sensitive cells and drug resistant cells after using drug.The change of VPA resistance in HL-60/HT cells was determinated simultaneously.The cell cycle of seneitive HL-60 cells and HL-60/HT cells were detected by flow cytometry.ResultsVPA in combination with Ara-C can obviously inhibit the growth of HL-60 and HL-60/HT cell,and the inhibition rate was higher than that of VPA or Ara-C alone(q=1.37,1.51).ConclusionVPA can inhibit the growth of HL-60 and HL-60/HT cell.It can play a synergistic role with Ara-C and reduce the drug resistance of leukemia cells.
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