抗癌烷化剂三溴基丙酮酸对秀丽隐杆线虫衰老指标及寿命的影响

摘要
图/表
参考文献(23)
相关文章 (1)

全文: PDF (0 KB) HTML (39 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨不同浓度的三溴基丙酮酸（3-BrPA）对秀丽隐杆线虫寿命的作用以及相关衰老指标的测试，系统评估其对生命状态的影响。方法选取同期化的秀丽隐杆线虫作为实验对象，选择3.125、6.250、12.500、25.000mmol/L的3-Br PA作为实验组，0.000mmol/L作为对照组。线虫寿命实验中，每个浓度均选择135条线虫，记录并绘制各个实验组和对照组的生存曲线；线虫吞咽能力实验每组均选择10条线虫，记录并绘制反映线虫衰老程度的吞咽速率图。结果秀丽隐杆线虫的寿命实验和吞咽能力实验结果均显示，3.125、25.000mmol/L实验组低于对照组，差异有统计学意义（P＜0.05）；而6.250、12.500mmol/L实验组与对照组比较，差异无统计学意义（P＞0.05）。结论 3.125、25.000mmol/L浓度的3-Br PA对秀丽隐杆线虫显示一定程度的损伤；而6.250、12.500mmo l/L浓度的3-BrPA对秀丽隐杆线虫并没有显示明显的毒副作用，且与对照组线虫相比，其线虫寿命并未缩短。产生此特殊现象或许和秀丽隐杆线虫体内丙酮酸浓度及饮食限制（DR）机制有关，具体原因仍在进一步研究。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	魏倩囡
	王素娟
	刘佳奇
	卜晓波
	李东航

关键词 ：抗癌烷化剂, 三溴基丙酮酸, 秀丽隐杆线虫, 抗衰老, 毒副作用

Abstract：Objective To explores the influence of different concentrations of 3-Bromopyruvate (3-BrPA)on the lifespan of Caenorhabditis elegans and tests of related aging indicators to systematically assess its impact on life conditions.Methods The same period of Caenorhabditis elegans was selected as the experimental object,3-BrPA of 3.125,6.250,12.500 and 25.000 mmol/L were selected as the experimental group,and 0.000 mmol/L were selected as the control group.A total of 135cases of Caenorhabditis elegans were selected for each concentration in the nematode life test group,and the survival curves of the each experimental group and the control group were recorded and plotted in the nematode swallowing capacity experiment,10 cases of Caenorhabditis elegans were selected in the each group,and the swallowing rate graph reflecting the aging degree of the Caenorhabditis elegans was recorded and plotted.Results Both Caenorhabditis elegans lifespan test and pharynx pumping ability test results showed that which in the experimental group of 3-BrPA of 3.125,25.000mmol/L were lower than those of the control group,and the differenceswere statistically significant(P＜0.05).There were no significant differences in the Caenorhabditis elegans lifespan test and pharynx pumping ability test results between the the experimental group of 3-BrPA of 6.250,12.500 mmol/L and the control group(P＞0.05).Conclusion 3-BrPA at 3.125and 25.000mmol/L showed a certain degree of damage to Caenorhabditis elegans;However,3-BrPA at the concentrations of 6.250 and 12.500mmol/L aren′t show significant toxic and side effect on Caenorhabditis elegans,and compared with the control group,the lifespan of the Caenorhabditis elegans is not shortened and prolonged.The occurrence of this special phenomenon may be related to the pyruvate concentration in the body of Caenorhabditis elegans and themechanism of dietary restriction (DR).The specific reasons are still being further studied.

Key words： Anticancer alkylating Agent 3-Bromopyruvate Caenorhabditis elegans Anti-aging Toxic side effect

基金资助:国家自然科学基金资助项目（81470062）

引用本文:

魏倩囡; 王素娟; 刘佳奇; 卜晓波; 李东航. 抗癌烷化剂三溴基丙酮酸对秀丽隐杆线虫衰老指标及寿命的影响[J]. 中国当代医药, 2020, 27(17): 4-7.
WEI Qian-nan; WANG Su-juan; LIU Jia-qi; BU Xiao-bo; LI Dong-hang. Influence of anticancer alkylating agent 3-Bromopyruvate on aging indexes and lifespan of Caenorhabditis elegans. 中国当代医药, 2020, 27(17): 4-7.

链接本文:

http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2020/V27/I17/4

[1]	中国肿瘤临床与康复编辑部.2017年中国最新癌症数据[J].中国肿瘤临床与康复，2017，24（6）：760.
[2]	葛瑶，任婷，崔鑫，等.新型多功能抗癌烷化剂研究进展[J].化学试剂，2018，40（8）：707-713，720.
[12]	Pichla M，Sroka J，Pienkowska N，et al.Metastatic prostate cancer cells are highly sensitive to 3-bromopyruvic acid[J].Life Sci，2019，227：212-223.
[18]	付喜梅.3-溴丙酮酸对秀丽隐杆线虫毒性和代谢的研究[D].兰州：兰州大学，2016.
[20]	Masoro EJ.Overview of caloric restriction and ageing[J].Mech Ageing Dev，2005，126（9）：913-922.
[3]	Chen TC，Yu J，Nouri Nigjeh E，et al.A perillyl alcoholconjugated analog of 3-bromopyruvate without cellular uptake dependency onmonocarboxylate transporter 1and with activity in 3-BP-resistant tumor cells[J].Cancer Lett，2017，400：161-174.
[4]	Yadav S，Pandey SK，Kumar A，et al.Singh antitumor and chemosensitizing action of 3-bromopyruvate：implication of deregulatedmetabolism[J].Chem Biol Interact，2017，270：73-89.
[5]	Sheng Y，Jiang Q，Dong X，et al.3-Bromopyruvate inhibits themalignant phenotype ofmalignantly transformed macrophages and dendritic cells induced by glioma stem cells in the gliomamicroenvironment[J].Biomed Pharmacother，2020，121：109610.
[6]	Xian SL，Cao W，Zhang XD，et al.3-Bromopyruvate inhibits human gastric cancer tumor growth in nudemice via the inhibition of glycolysis[J].Oncol Lett，2015，9（2）：739-744．
[7]	Huang RT，Huang Q，Wu GL，et al.Evaluation of the antioxidant property and effects in Caenorhabditis elegans of Xiangxi flavor vinegar，a hunan local traditional vinegar[J].Zhejiang Univ Sci B，2017，18（4）：324-333.
[8]	García-EspineiraMC，Tejeda-Benítez LP，Olivero-Verbel J.Toxic effects of bisphenol A，propyl paraben，and triclosan on Caenorhabditiselegans[J].Int JEnviron Res Public Health，2018，15（4）：684.
[9]	Gu QL，Zhang Y，Fu XM，et al.Toxicity and metabolism of 3-bromopyruvate in Caenorhabditis elegans[J].JZhejiang Univ Sci B，2020，21（1）：77-86.
[10]	Yadav S，Pandey SK，Goel Y，et al.Diverse stakeholders of tumor metabolism：an appraisal of the emerging approach ofmultifaceted metabolic targeting by 3-bromopyruvate[J].Front Pharmacol，2019，10：728.
[11]	Wood JG，Rogina B，Lavu S，et al.Sirtuin activatorsmimic caloric restriction and delay ageing inmetazoans[J].Nature，2004，430（7000）：686-689.
[13]	Cho EJ，Yu SJ，Kim K，et al.Carbonic anhydrase-Ⅸinhibition enhances the efficacy of hexokinaseⅡinhibitor for hepatocellular carcinoma in a murine model[J].JBioenerg Biomembr，2019，51（2）：121-129.
[14]	Yamada M，Kagaya M，Noguchi N，et al.Topical 3-bromopyruvate is a novel targeted therapy formelanoma in a preclinicalmodel[J].JDermatol Sci，2018，92（2）：134-142.
[15]	Yoo JJ，Yu SJ，Na J，et al.Hexokinase-Ⅱinhibition synergistically augments the anti-tumor efficacy of sorafenib in hepatocellular carcinoma[J].Int JMol Sci，2019，20（6）：1292.
[16]	Ishiguro Y，Kobayashi M，Ideno M，et al.Valproate sensitizeshuman glioblastoma cells to 3-bromopyruvate-induced cytotoxicity[J].Int JPharm，2018，551（1-2）：97-102.
[17]	Chiasserini D，Davidescu M，Orvietani PL，et al.3-Bromopyruvate treatment induces alterations ofmetabolic and stress-related pathways in glioblastoma cells[J].J Proteomics，2017，152：329-338.
[19]	Mouchiroud L，Molin L，Kasturi P，et al.Pyruvate imbalancemediatesmetabolic reprogramming and mimics lifespan extension by dietary restriction in Caenorhabditis elegans[J].Aging Cell，2011，10（1）：39-54.
[21]	Maeda H，Gleiser CA，Masoro EJ，et al.Nutritional inflfluenceson aging of Fischer 344rats：Ⅱ.Pathology[J].JGerontol，1985，40（6）：671-688.
[22]	Lane MA，Ingram DK，Roth GS.Calorie restriction in nonhuman primates：effects on diabetes and cardiovascular disease risk[J].Toxicol Sci，1999，52（2）：41-48.
[23]	Mattson MP，Wan R.Benefificial effects of intermittent fasting and caloric restriction on the cardiovascular and cerebrovascular systems[J].Nutr Biochem，2005，16（3）：129-137.