骨髓基质细胞自体移植兔坐骨神经的研究

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摘要目的观察兔自体骨髓基质细胞作为种子细胞移植修复兔坐骨神经长距离缺损的能力及其安全性，探讨骨髓基质细胞在坐骨神经损伤模型内分化为神经元样细胞的可行性。方法兔骨髓基质细胞分离培养、诱导、鉴定以及传代后注入坐骨神经损伤的兔模型中，并于细胞移植后第1、2、4、8及16周分别行透射电镜及免疫组化等方法观察。结果移植侧与对照侧神经纤维均有不同程度再生，但对照侧较移植侧神经纤维数量明显减少（t=175.88，P＜0.01）。结论BMCSs移植能促进兔坐骨神经功能障碍的恢复，并与本身相容性良好，为细胞替代疗法提供了乐观的应用前景。

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	何骁
	邓莉莉

关键词 ：骨髓基质细胞, 细胞培养, 自体移植, 坐骨神经

Abstract：Objective To observe the ability and safety of autologous bone marrow stromal cells(BMSCs)as seed cells in repairing long distance defect of sciatic nerve in rabbits,and to explore the feasibility of BMSCs differentiating into neuron-like cells in sciatic nerve injury model.Methods BMSCs were injected into rabbit model of sciatic nerve injury, after isolated,cultured,induced,and identificated.Then electron microscope and immunohistochemisty were performed at the 1st,2nd,4th,8thand 16thweeks after cell transplantation.Results After transplantation,there were different degrees of regenerated nerve fibers between transplanted side and controlled side,but the number of nerve fibers was significantly reduced in control group,compared with the transplantation group(t=175.88,P＜0.01).Conclusion BMCSs transplantation has a well compatibility,which can promote the recovery of rabbit sciatic nerve dysfunction and provide an optimistic application prospect for cell replacement therapy.

Key words： Bone marrow stromal cell Cell culture Autotransplantation Sciatic nerve

作者简介: 何骁（1982-），男，湖南郴州人，硕士，主治医师，主要从事关节、创伤相关疾病的研究

引用本文:

何骁；邓莉莉. 骨髓基质细胞自体移植兔坐骨神经的研究[J]. 中国当代医药, 2017, 24(3): 13-16.
HE Xiao；DENG Li-li. Research on bone marrow stromal cells autotransplantation into rabbit′s sciatic nerve. 中国当代医药, 2017, 24(3): 13-16.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2017/V24/I3/13

[2]	石更强，胡益.周围神经种子细胞的研究进展[J].生物医学工程学杂志，2015，32（2）：470-474.
[4]	姚东东，张洁元，刘彬.周围神经损伤修复微环境的研究进展[J].中国修复重建外科杂志，2015，29（9）：1167-1170.
[11]	方波，马虹.骨髓基质干细胞移植治疗脊髓损伤机制的研究进展[J].中国康复医学杂志，2011，26（10）：985-989.
[1]	Kim HJ，Lee JH，Kim SH，et al.Therapeutic effects of human mesenchymal stem cells on traumatic brain injury in rats：secretion of neurotrophic afators and inhibition of apoptosis[J]. J Neurotrauma，2010，27（l）：131-138.
[3]	Torres RY，Miranda GE.Epidemiology of traumatic peripheral nerve injuries evaluated by electrodiagnostic studies in a tertiary care hospital clinic[J].Bol Asoc Med P R，2015，107（3）：79-84.
[5]	Höke A.Mechanisms of disease：what factors limit the success of peripheral nerve regeneration in humans[J].Nat Clin Pract Neurol，2006，2（8）：448-454.
[6]	Wakao S，Matsuse D，Dezawa M.Mesenchymal stem cells as a source of Schwann cells：their anticipated use in peripheral nerve regeneration[J].Cell Tissues Organs，2014，200（1）：31-41.
[7]	MohammadiR，MahmoodzadehS.Combinationoflocal transplantation of in vitro bone-marrow stromal cells and pulsed electromagnetic fields accelerate functional recovery of transected sciatic nerve regeneration：a novel approach in transected nerve repair[J].Curr Neurovasc Res，2015，12（3）：222-231.
[8]	Hu J，Zhu QT，Liu XL，et al.Repair of extended peripheral nerve lesions in rhesus monkeys using acellular allogenic nerve grafts implanted with autologous mesenchymal stem cells[J].Exp Neurol，2007，204（2）：658-666.
[9]	Gong Y，Wang H，Xia H.Stable transfection into rat bone marrow mesenchymal stem cells by lentivirus-mediated NT-3[J].Mol Med Rep，2015，11（1）：367-373.
[12]	Chopp M，Li Y.Treatment of neural injury with marrow stromal cells[J].Lancet Neurol，2002，1（2）：92-100.
[13]	]Totey S，Totey S，Pal R，et al.Adult stem cells：a clinical update[J].Stem Cells，2009，4（2）：105-121.
[17]	Osakada F，Hirami Y，Takahashi M.Stem cell biology and cell transplantation therapy in the retina[J].Biotechnol Genet Eng Rev，2010，26：297-334.
[10]	Freedman MS，Bar-Or A，Atkins HL，et al.The therapeutic potential of mesenchymal stem cell transplantation as a treatment for multiple sclerosis：consensus report of the International MSCT Study Group[J].Mult Scler，2010，16（4）：503-510.
[14]	Hou SY，Zhang HY，Quan DP，et al.Tissue-engineered peripheral nerve grafting by diffentiated bone marrow stromal cells[J].Neuroscience，2006，140（1）：101-110.
[15]	Salomone R，Bento RF，Costa HJ.Bone marrow stem cells in facial nerve regeneration from isolated stumps[J].Muscle Nerve，2013，48（3）：423-429.
[16]	Schafer KH，Micci MA，Pasricha PJ.Neural stem cell transplantation in the enteric nervous system：roadmaps and roadblocks[J].Neurogastroenterol Motil，2009，21（2）：103-112.
[18]	Cunullings BJ，Tamaki SJ.Human neural stern cells differentiate and promote locomotor recover in spinal cord-injured mice[J].Proc Natl Acad Sci U S A，2005，102（39）：1406-1407.
[19]	Mohammadi R，Vahabzadeh B，Amini K.Sciatic nerve regeneration induced by transplantation of in vitro bone marrow stromal cells into an inside-out artery graft in rat[J]. Craniomaxillofac Surg，2014，42（7）：1389-1396.