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Effect analysis of quantitative count of bacteria in urinary sediments on screening of urinary system infection |
YE Li-yaMA Cai-yunLU Feng-quanWU Ming |
Department of Laboratory,Shengze Hospital Affiliated to Nanjing Medical University,Jiangsu Province,Suzhou 215228,China |
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Abstract Objective To explore the effect of quantitative count of bacteria in urinary sediments on screening of urinary system infection.Methods The urine samples of 400 patients with urinary tract infection were collected from Shengze Hospital Affiliated to Nanjing Medical University from January 2017 to December 2019 were selected as the research subjects,all patients were collected middle urine and carried out urine bacterial culture and quantitative count of bacteria in urinary sediments.Using the urine bacterial culture results as the gold standard,the sensitivity,specificity,accuracy and false positive rate of quantitative counting of urine sediment bacteria were observed.Results Among 400 specimens,the positive rate of urine bacteria culture was 50.50%(202/400),and the positive rate of quantitative count of urine sediment bacteria was 51.25%(205/400),two methods were consistent in positive detection rate(Kappa value=0.865).Using urine bacteria culture as the gold standard,the quantitative count of bacteria in urinary sediments was 79.21%(160/202),the specificity was 77.27%(153/198),the accuracy was 78.25%(313/400),and the false positive rate was 21.95%(45/205).The accuracy of quantitative count of bacteria in urinary sediments in the diagnosis of Gram-negative bacteria was 73.50%(294/400),the sensitivity was 67.31%(105/156),and the specificity was 74.41%(189/254);the accuracy in the diagnosis of Gram-positive bacteria was 92.00%(368/400),the sensitivity was 72.00%(36/50),the specificity was 94.86%(332/350);the accuracy in the diagnosis of chlamydia was 94.00%(376/400),the sensitivity was 66.67%(4/6),the specificity was 94.42%(372/394).Conclusion Although urine bacterial culture and urinary sediment quantitative counting has fair positive detection rate,urinary sediment quantitative counting still can not be replaced and may be considered as early screening indicators of urinary system infection.
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