不同剂量银杏叶提取物对野百合碱所致肺动脉高压的作用效果研究

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Abstract Objective To study the effect of different doses of Ginkgo biloba extract (GBE) in monocrotaline-induced pulmonary arterial hypertension, and to measure the expression of endothelin-1 (ET-1) and nitric oxide synthase(eNOS) in lung tissue. Methods According to the random number table method, 50 male SD rats were divide into five groups: normal control group (group C), pulmonary arterial hypertension model group (group M), GBE low-dose treatment group(group G1,40 mg/kg),GBE medium-dose treatment group(group G2,60 mg/kg)and the GBE high-dose treatment group (group G3, 80 mg/kg), 10 rats in each group. The right atrial pressure (RAP), right ventricular systolic pressure (RVSP), mean pulmonary artery pressure (mPAP) and right ventricular hypertrophy index (RVHI) in rats were measured. The histological structure of small pulmonary arteries was observed by HE staining, and the percentage of mesothelial thickness to the outer diameter of the vessel (MA%) and ratio of the mesothelial area to the total area of the vessel (MV%) for 50-150 μm diameter pulmonary arteries were calculated. The expression of ET-1 and eNOS in lung tissue of rats was determined by immunohistochemistry. Results Compared with the group M, the RAP, mPAH, RVSP,RVHI, MA% and MV% of the rats in the three treatment groups decreased, and the differences were statistically significant (P<0.05). Among the three treatment groups, RAP, mPAH and MV% in group G3 were statistically significant compared with group G1 (P<0.05). In eNOS, the expression of eNOS in five groups: group C>group G3>group G1>group G2>group M, the expression levels of eNOS in the three treatment groups were higher than those in the group M, and the differences were statistically significant (P<0.05). The expression of eNOS in the G3 group was compared with the group G1 and group G2, and the differences were statistically significant (P<0.05). In ET-1, the expression of ET-1 in five groups: group M>group G1>group G2>group G3>group C, the expression levels of ET-1 in the three treatment groups were lower than those in the group M, and the differences were statistically significant (P<0.05). The expression of ET-1 in the G3 group was compared with the group G1 and group G2, and the differences were statistically significant (P<0.05). Conclusion GBE can delay the formation of pulmonary arterial hypertension, which is related to inhibiting the release of ET-1 and maintaining the synthesis of eNOS. Compared with low and medium doses, high doses of GEB could enhance the delaying effect formation of pulmonary arterial hypertension in a dose-dependent manner.

Key words： Ginkgo biloba extract Monocrotaline Pulmonary arterial hypertension Nitric oxide synthase Endothelin-1

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	SHEN Tao
	WU Xu
	LU Zhu-ming

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SHEN Tao,WU Xu,LU Zhu-ming. Study on the effect of different doses of Ginkgo biloba extract on monocrotaline-induced pulmonary arterial hypertension[J]. 中国当代医药, 2021, 28(16): 12-16.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I16/12

[1]	刘蕾，马壮.肺动脉高压诊治指南解读[J].中国实用内科杂志，2020，40（5）：377-381.
[2]	Xu D，Li Y，Zhang B，et al.Resveratrol alleviate hypoxic pulmonary hypertension via anti-inflammation and anti-oxidant pathways in rats[J].Int J Med Sci，2016，13（12）：942-954.
[3]	Nogueira-Ferreira R，Vitorino R，Ferreira R，et al.Exploring the monocrotaline animal model for the study of pulmonary arterial hypertension：A network approach[J].Pulm Pharmacol Ther，2015，35：8-16.
[4]	Davies RJ，Holmes AM，Deighton J，et al.BMP type Ⅱreceptor deficiency confers resistance to growth inhibition by TGF-beta in pulmonary artery smooth muscle cells：role of proinflammatory cytokines[J].Am J Physiol Lung Cell Mol Physiol，2012，302（6）：L604-L615.
[5]	Benza RL，Miller DP，Barst RJ，et al.An evaluation of longterm survival from time of diagnosis in pulmonary arterial hypertension from the REVEAL Registry[J].Chest，2012，142（2）：448-456.
[6]	陈建，何冰，刘新民，等.银杏叶制剂治疗肺间质纤维化的实验研究[J].中国中西医结志，2000，20（6）：441.
[7]	沈涛，吴旭，张振中，等.银杏叶提取物对野百合碱所致肺动脉高压保护作用的实验研究[J].感染、炎症、修复，2014，15（1）：31-35，65.
[8]	孙波，刘文利.右心导管测定大鼠肺动脉压的实验方法[J].中国医学科学院学报，1984，6（6）：465-467.
[13]	谭玉萍，王朝晖，蒙定水，等.银杏叶提取物对肺气肿大鼠血管内皮生长因子的影响[J].广西医科大学学报，2010，27（2）：208-210.
[9]	Rahman MA，Dhar DK，Yamaguchi E，et al.Coexpression of inducible nitric oxide synthase and COX-2 in hepatocellular carcinoma and surrounding liver：possible involvement of COX-2 in the angiogenesis of hepatitis C virus-positive cases[J].Clin Cancer Res，2001，7（5）：1325-1332.
[10]	Hessel MH，Steendijk P，den Adel B，et al.Characterization of right ventricular function after monocrotaline-induced pulmonary hypertension in the intact rat[J].Am J Physiol Heart Circ Physiol，2006，291（5）：H2424-H2430.
[11]	Lee H，Yeom A，Kim KC，et al.Effect of ambrisentan therapy on the expression of endothelin receptor，endothelial nitricoxidesynthaseandNADPHoxidase4inmonocrotalineinduced pulmonary arterial hypertension rat model[J].Korean Circ J，2019，49（9）：866-876.
[12]	Liu F，Zhang J，Yu S，et al.Inhibitory effect of Ginkgo biloba extract on hyperhomocysteinemia-induced intimal thickening in rabbit abdominal aorta after balloon injury[J].Phytother Res，2008，22（4）：506-510.
[14]	Wei JM，Wang X，Gong H，et al.Ginkgo suppresses atherosclerosis through downregulating the expression of connexin 43 in rabbits[J].Arch Med Sci，2013，9（2）：340-346.
[15]	Craig JC，Broxterman RM，La Salle DT，et al.The role of endothelin A receptors in peripheral vascular control at rest and during exercise in patients with hypertension[J].J Physiol，2020，598（1）：71-84.
[16]	Jigisha P，Susan H，Lucie C，et al.Counteracting effects of treprostinil and endothelin （ET-1） receptor antagonists（ETRAs）on endothelin-1，ETB receptor and ECE-1 levels in pulmonary smooth muscle cells （PASMCs） derived from patients with pulmonary arterial hypertension （PAH）[J].Life Sci，2013，93（25-26）：e43-e44.
[17]	Guo Q，Xu H，Yang X，et al.Notch activation of Ca2+-sensing receptor mediates hypoxia-induced pulmonary hypertension[J].Hypertens Res，2017，40（2）：117-129.
[18]	Chester AH，Yacoub MH.The role of endothelin-1 in pulmonary arterial hypertension[J].Glob Cardiol Sci Pract，2017，2014（2）：62-78.
[19]	Racke K，Fuhrmann M，Juergens UR，et al.Over expression of endothelin-1 （ET-1）in lung fibroblasts （LFb）from patients with pulmonary arterial hypertension （PAH），evidence for loss of inhibitory control[J].Eur Respir J，2016，48（Suppl 60）：PA1820.
[20]	Wang Z，Li AY，Guo QH，et al.Effects of cyclic intermittent hypoxia on ET-1 responsiveness and endothelial dysfunction of pulmonary arteries in rats[J].PLoS One，2013，8（3）：e58078.
[21]	Bredmose PP，Buskop C，Lmo AB.Inhaled nitric oxide might be a contributing tool for successful resuscitation of cardiac arrest related to pulmonary hypertension[J].Scand J Trauma Resusc Emerg Med，2019，27（1）：22.
[22]	Wang K，Kessler PD，Zhou Z，et al.Local adenoviral-mediated inducible nitric oxide synthase gene transfer inhibits neointimal formation in the porcine coronary stented model[J].Mol Ther，2003，7（5 Pt 1）：597-603.