基于网络药理学的白头翁治疗肺结核作用机制研究

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Abstract Objective To screen the active constituents of Pulsatilliae Radix based on the method of network pharmacology,to predict the potential therapeutic targets of pulmonary tuberculosis and to explore its potential mechanism.Methods Pulsatilliae Radix ingredients,corresponding targets and target genes related to the treatment of pulmonary tuberculosis were selected from the traditional Chinese medicine systems pharmacology database and analysis platform,PubChem,Pharm Mapper,Uniprot,Gene Cards,OMIM databases.Combined with the String database and Cytoscape 3.6.1 software,the active component-target network diagram and the protein-protein interaction network for Pulsatilliae Radix tuberculosis treatment were constructed.GO enrichment analysis and KEGG signal pathway enrichment analysis of Pulsatilliae Radix tuberculosis treatment targets were performed by DAVID database and Weishengxin online mapping software.Results Using oral bioavailability and drug-like parameters as criteria,the 9 herb ingredients such as Mairin,Aureusidin,β-sitosterol,isorhamnetin,Stigmasterol, etc,were screened out.It is mainly used to treat tuberculosis by regulating potential targets such as nitric oxide synthase 2,leukotriene A4 hydrolase,peroxisome proliferators activate receptors, etc,these targets may through pathways in cancer and tumor necrosis factor signaling pathway to play to the role of the treatment of pulmonary tuberculosis.Conclusion Therefore,through the network pharmacological research,it was preliminarily predicted that Stigmasterol might be the main active ingredient of Pulsatilliae Radix in the treatment of pulmonary tuberculosis by regulating the cancer signal pathway and tumor necrosis factor signaling pathway,activating the process of apoptosis,participating in the immune response, etc,so as to make Mycobacterium tuberculosis lose living environment,play the role of treating tuberculosis thereby.

Key words： Pulsatilliae Radix Pulmonary tuberculosis Network pharmacology Mechanism

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	LIU Yan-xia
	REN Ming-hui
	DU Bao-zhong

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LIU Yan-xia,REN Ming-hui,DU Bao-zhong. Study on the mechanism of Pulsatilliae Radix in the treatment of tuberculosis based on network pharmacology[J]. 中国当代医药, 2021, 28(27): 4-9.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I27/4

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