肝细胞癌组织中微小RNA-552、微小RNA-383的表达及其对患者术后5年不良预后结局的影响

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Abstract Objective To detect the expression of microRNA-552 (miR-552) and microRNA-383 (miR-383) in hepatocellular carcinoma (HCC) and to investigate the effect of miR-552 and miR-383 on the prognosis of HCC patients.Methods From February 2011 to February 2014, 68 patients with HCC who were hospitalized and underwent radical resection in Huadu District People′s Hospital of Guangzhou City were selected, the HCC tissue of the patients was taken as the study sample, and the adjacent normal tissue(more than 5 cm from the edge of the tumor) was taken as the control sample, the relative expression of miR-552 and miR-383 was detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). According to the relative expression of miR-552 and miR-383 in HCC tissues, the patients were divided into miR-552 low expression group (miR-552 relative expression <1.28, n=26), miR-552 high expression group (miR-552 relative expression >1.28, n=42), miR-383 low expression group (miR-383 relative expression <0.83, n=29) and miR-383 high expression group (miR-383 relative expression >0.83, n=39). χ2 test was used to analyze the relationship between the expression of miR-552 and miR-383 and the clinicopathological characteristics of HCC. Kaplan-Meier survival curve was used to analyze the factors influencing the prognosis of HCC, and COX regression was used to analyze the factors influencing the 5-year postoperative outcome of HCC. Results The relative expression of miR-552 in HCC tissues was higher than that in adjacent normal tissues, the difference was statistically significant (P<0.05), the relative expression of miR-383 was lower than that in adjacent normal tissues, the difference was statistically significant (P<0.05); comparison of age, gender, the proportion of liver cirrhosis between miR-552 high expression group and miR-552 low expression group, the differences were not statistically significant (P>0.05), while the proportions of high differentiation, tumor diameter ≥5 cm, lymph node metastasis, distant metastasis, TNM stage Ⅲ-Ⅳand vascular invasion in miR-552 low expression group were lower than those in miR-552 high expression group, the differences were statistically significant(P<0.05); comparison of age, gender, proportions of liver cirrhosis, distant metastasis and vascular invasion between miR-383 low expression group and miR-383 high expression group, the differences were not statistically significant (P>0.05), while the proportions of high differentiation, tumor diameter ≥5 cm, lymph node metastasis and TNM stage Ⅲ-Ⅳin miR-383 low expression group were higher than those in miR-383 high expression group, the differences were statistically significant (P<0.05);the 5-year survival rate of miR-552 low expression group was higher than that of miR-552 high expression group, the difference was statistically significant (P<0.05), and the 5-year survival rate of miR-383 low expression group was lower than that of miR-383 high expression group, the difference was statistically significant(P<0.05). The high expression of miR-552 was an independent risk factor for poor prognosis 5 years after surgery (β=0.558, HR=1.748, 95%CI=1.065-2.870, P<0.05). The high expression of miR-383 was an independent protective factor for the poor prognosis 5 years after surgery (β=-0.458, HR=0.634, 95%CI=0.467-0.861, P<0.05). Conclusion The expression of miR-552 and miR-383 in HCC is increased and decreased respectively, both of them are significantly related to 5-year survival rate of HCC patients, which may have some value in judging the prognosis of HCC patients.

Key words： Hepatocellular carcinoma microRNA-552 microRNA-383 Prognosis

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	Articles by authors
	ZHAO Juan
	LI Hui
	XU Pei-yan2
	ZHANG Cheng-fang
	DU Li-jun
	CAO Tian-sheng

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ZHAO Juan,LI Hui,XU Pei-yan2, et al. Expression of microRNA-552 and microRNA-383 in hepatocellular carcinoma and their influence on 5-year poor prognostic outcome[J]. 中国当代医药, 2021, 28(21): 23-28.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I21/23

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