基于1H-NMR技术的慢性肾衰竭代偿期气阴两虚证血清代谢分析

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Abstract Objective To analyze the serum metabolism of qi and yin deficiency syndrome in the compensated stage of chronic renal failure based on 1H-NMR technology. Methods From January to November 2019, 15 voluntary patients with qi and yin deficiency syndrome in the compensated stage of chronic renal failure admitted to the Third Department of Nephrology, Shanxi Hospital of Integrated Traditional and Western Medicine were selected as the disease group, and 15 healthy patients′ family members and volunteers were selected as the control group. Fasting venous blood was collected and prepared for sample processing. 1H-NMR technology combined with principal component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA) and other diversified analytical methods were used to find endogenous compounds and compare metabolite differences between groups. Results In the disease group, the contents of seven metabolic differences including arginine, proline and lipid were higher than those in the control group,and the contents of two kinds of choline and phosphatidylcholine were lower than those in the control group, the differences were statistically significant (P＜0.05). Conclusion It is initially found that there is a specific metabolic profile in the syndrome of deficiency of both qi and yin in the compensatory phase of chronic renal failure. The metabolic disorder of qi and yin deficiency syndrome in the compensatory phase of CRF may mainly involve amino acid and lipid metabolism. Metabonomics technology can provide a more comprehensive reference for the modern research and clinical practice of traditional Chinese medicine kidney disease,and help realize the ultra-early diagnosis and treatment of chronic renal failure.

Key words： Chronic renal failure Chronic kidney disease Metabolomics; 1H -NMR Multivariate statistical analysis Deficiency of both qi and yin

Received: 25 November 2020

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[3]	van der Weerd NC，Grooteman MP，Nubé MJ，et al.Hepcidin in chronic kidney disease：not an anaemia management tool，but promising as a cardiovascular biomarker[J].Neth J Med，2015，73（3）：108-118.
[8]	牛倩芸，刘月涛，李震宇，等.基于核磁代谢组学技术的阿霉素肝毒性研究[J].药学学报，2015，50（6）：708-713.
[9]	国家中医药管理局医政司.22 个专业95 个病种中医诊疗方案—慢性肾衰（慢性肾功能衰竭）诊疗方案[M].北京：中医药管理局医政司，2010：203-208．
[10]	卢嫣，苏哲苓，杨莉，等.肾系疾病肝肾阴虚证血液代谢组学初探[J].上海中医药杂志，2015，49（3）：10-13.
[11]	Mikami D，Kobayashi M，Uwada J，et al.β-Hydroxybutyrate enhances the cytotoxic effect of cisplatin via the inhibition of HDAC/survivin axis in human hepatocellular carcinoma cells[J].J Pharmacol Sci，2020，142（1）：1-8.
[12]	洪宇.慢性肾脏病患者血脂与血糖的调节[C].中国中西医结合学会肾脏疾病专业委员会.中国中西医结合学会肾脏疾病专业委员会2018年学术年会专题讲座汇编.中国中西医结合学会肾脏疾病专业委员会：中国中西医结合学会，2018：246-247.
[13]	康世宗，张海臣，吴永辉，等.L-α-甘油磷脂酰胆碱的制备、纯化及检测研究进展[J].中国油脂，2017，42（8）：103-107.
[14]	李亚敏.基于网络药理学和多组学的细辛肺毒性机制研究[D].武汉：湖北中医药大学，2019.
[15]	陈肖，阮一，何瑶，等.精氨酸强化的肠内营养对创伤患者预后及免疫功能影响的Meta 分析[J].中国药房，2019，30（10）：1418-1423.
[16]	欧阳暂.精氨酸强化的免疫营养支持对胃癌患者术后免疫功能影响的Meta 分析[D].重庆：重庆医科大学，2018.
[17]	金薇，潘萍萍，袁新华.精氨酸、左旋多巴单日复合与两日序贯激发生长激素分泌的效果评价[J].中国儿童保健杂志，2011，19（7）：651-652.
[18]	张敏继，高继宁，王洋.高继宁教授治疗慢性肾脏病2～3 期临证经验[J].中国医药导报，2020，17（17）：158-161.
[1]	中华医学会.临床诊疗指南·肾脏病学分册[M].第1 版.北京：人民卫生出版社，2017：218.
[2]	Lv JC，Zhang LX.Prevalence and Disease Burden of Chronic Kidney Disease[J].Adv Exp Med Biol，2019，1165：3-15.
[4]	赵德龙.维持性血液透析患者流行病学调查及生存预后相关性分析[D].北京：中国人民解放军医学院，2016．
[5]	李跃进，高继宁，沈辛宜，等.慢性肾功能衰竭早中期脾肾气虚证患者血清代谢组学浅析[J].中国药物与临床，2017，17（5）：733-735.
[6]	高继宁，任云城，沈丽萍，等.慢性肾衰竭早中期脾肾气虚证患者尿液代谢组学研究[J].山西中医，2017，33（11）：42-46.
[7]	何立群.慢性肾衰竭的诊断、辨证分型及疗效评定（试行方案）[J].上海中医药杂志，2006，（8）：8-9.