5种尿液修饰核苷联合血浆EBV-DNA定量检测在鼻咽癌诊断中的可行性研究

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Abstract Objective To explore the feasibility of combined detection of five urinary modified nucleosides and plasma EBV-DNA in the diagnosis of nasopharyngeal carcinoma.Methods A total of 56 patients with nasopharyngeal carcinoma treated in Zhejiang Cancer Hospital from January to June 2020 were selected as the nasopharyngeal carcinoma group and 32 healthy subjects during the same period were selected as the healthy control group.Liquid chromatography-tandem mass spectrometry was employed to measure the concentrations of modified nucleosides pseudouridine(Pseu),2-O-methylguanosine(m2G),1-methyladenosine(m1A),6-methyladenosine(m6A)and 1-methylguanosine(m1G)in urine samples.The levels of Pseu,m2G,m1A,m6A and m1G in urine samples of the two groups were compared.Fluorescent quantitative polymerase chain reaction was employed to measure the level of the plasma EBV-DNA in peripheral blood samples collected from all the subjects.Receiver operating characteristic(ROC)curve was made to evaluate the diagnostic value of single detection and combined detection of urinary nucleosides and plasma EBV-DNA.Results The levels of Pseu,m2G,m1A and m1G in the nasopharyngeal carcinoma group were higher than those in the healthy control group,the differences were statistically significant(P＜0.05).The level of the plasma EBV-DNA in the nasopharyngeal carcinoma group was higher than that in the healthy control group,the difference was statistically significant(P＜0.05).The area under the curve(AUC)of the combined detection was higher than that of m2G,m1A,Pseu,m1G and EBV-DNA in individuals,the differences were statistically significant(P＜0.05).In the nasopharyngeal carcinoma patients,the Pseu concentration was positively correlated with clinical stage and lymph node metastasis(r=0.474,0.406,P＜0.01).Conclusion The urinary modified nucleosides Pseu,m2G,m1A and m1G may be small molecular markers for the diagnosis of nasopharyngeal carcinoma.The combined detection of the four urinary nucleosides with plasma EBV-DNA may help to improve the diagnostic efficiency of nasopharyngeal carcinoma.

Key words： Nasopharyngeal carcinoma Modified nucleosides EBV-DNA Liquid chromatography-tandem mass spectrometry

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	MAO Fei-xia
	ZHANG Yi-min
	ZHANG Jian-ying

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MAO Fei-xia,ZHANG Yi-min,ZHANG Jian-ying. Feasibility study of combined detection of five urinary modified nucleosides and plasma EBV-DNA in the diagnosis of nasopharyngeal carcinoma[J]. 中国当代医药, 2021, 28(14): 8-12.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I14/8

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