微柱凝胶法交叉配血次侧凝集原因分析及临床意义

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Abstract Objective To evaluate the agglulination reasons for the minor cross-matching,to provide reference for the safety of clinical blood transfusion.Methods Cases of patients with side crosss-matching agglulination were chosen from January 2013 to May 2016 in our hospital,according to the disease group,to investigate the correlation of the proportion of side cross-matching agglulination and the numbers of blood transfusion and the blood transfusion reaction.We had analysed the reasons of the side crossmatching agglulination by the antiglobulin test,antibodies identification,drug antibody screening and identification,and the size of RBC and morphology,combined with the clinical blood transfusion.Results 45 672 cases were investigated by micro-column gel way,ther were 325 cases side agglutination,the agglutination rate was 0.71%.The patients with side cross-matching agglulination mainly included haematology patients,ICU critical patients and emergency and burn patients.The agglulination rates of the haematology patients associated with the numbers of blood transfusions,there were positive correlation between the times of blood transfusion reaction and the times of blood transfusion(P＜0.05),but there were no relevance between the agglulination rates for the patients in the ICU and emergency room and the times of blood transfusion(P＞0.05).The reasons of side cross-matching agglulination mainly included,drugs antibodies,plasmas proteins,erythrocyte alloantibody of donor,the changes of the erythrocyte morphology,improper sample,the bad blood anticoagulation,the old red cell，insufficient washing cell,and so on.There were significant difference between the cases caused by immune and non immune factors in the side crosssmatching agglulination(P＜0.05).Conclusion The agglulination reasons of the side cross-matching have many types,the agglulination rates caused by non immune factors,are the highest in the clinical blood transfusion,which show low transfusion reaction rate and infusion inefficiency rate.But the side cross-matching agglulination cases caused by the imune factors easily lead to more transfusion reaction.and worse infusion efficiency.

Key words： Cross-matching Minor agglulination Reasons analysis Clinical significance

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	Articles by authors
	LI Miao-shan
	WU Chang-lin
	ZHOU Xue-min
	DANG Xin-tang
	ZHU Yi
	SHAO Chao-peng

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LI Miao-shan,WU Chang-lin,ZHOU Xue-min, et al. Study on the reasons of the Minor Cross-matching agglulination by Micro-column Gel way and its clinical significance[J]. 中国当代医药, 2017, 24(28): 14-17.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I28/14

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