NLRP3炎症小体的表达及多态性与急性冠脉综合征患者Caspase-1水平和冠脉病变程度的关系研究

Abstract
Figure/Table
References (15)
Related Citation (15)

Download: PDF (0 KB) HTML (36 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To study the relationship between the expression and polymorphism of inflammatory bodies of Nod like receptor protein 3 (NLRP3) and the level of Caspase-1 and the degree of coronary lesions in patients with acute coronary syndrome (ACS).Methods From January 2018 to may 2019,150 patients with ACS diagnosed clinically in the Department of Cardiology of the Second Affiliated Hospital of Shenyang Medical College were selected as the experimental group,50 patients suspected with coronary heart disease admitted to hospital during the same period but who underwent negative coronary angiography examination were selected as the control group.The peripheral blood was collected and the rs10754558 polymorphism of NLRP3 gene was detected.The serum was isolated and the activity index of inflammatory body was measured.Coronary angiography was performed at the same time.The expression levels of NLPR3 and Caspase-1 were compared between the two groups.According to the results of coronary angiography,the expression levels of NLPR3 and Caspase-1 and the Gensini scores of different coronary lesions were recorded.The coronary lesions of different NLRP3 genotypes were observed,and the relationship between the expression and polymorphism of NLRP3 and the level of Caspase-1 and the degree of coronary lesions were analyzed.Results The expression levels of NLPR3 and Caspase-1 in the experimental group were higher than those in the control group (P＜0.05).In the experimental group,the expression levels of NLPR3,Caspase-1 and Gensini score in the patients with three vessel lesions were higher than those in the patients with two vessel lesions and single vessel lesion,the expression levels of NLPR3,Caspase-1 and Gensini score in patients with two vessel lesions were higher than those in patients with single vessel lesion,the differences were statistically significant (P＜0.05).Pearson correlation analysis showed that NLPR3 expression level was positively correlated with Caspase-1 expression level and Gensini score (r=0.714,0.781,P＜0.05).Among the rs10754558 genotypes of NLRP3 gene in the experimental group,44 cases (29.33%)were CC genotype,46 cases (30.67%)were GG genotype,and 60 cases(40.00%) were GC genotype.In the rs10754558 genotypes of NLRP3 gene in the control group,40 cases (80.00%) were CC genotype,4 cases (8.00%) were GG genotype and 6 cases (12.00%) were GC genotype.The proportions of GG genotype and GC genotype in NLRP3 gene of the experimental group were higher than those of control group,and the proportion of CC genotype in NLRP3 gene of the experimental group was lower than that of control group,the differences were statistically significant (P＜0.05).In the experimental group,the three branches lesions were mostly seen in the GG and GC types of NLPR3.Conclusion The expression of NLPR3 in ACS patients is positively correlated with Caspase-1 expression level and Gensini score.The increase of allele g at rs10754558 of NLRP3 gene can increase the activity of inflammatory bodies,indicating the severity of coronary lesions and poor prognosis.

Key words： Acute coronary syndrome NLPR3 Inflammatory corpuscle Gene polymorphism

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WU Wei
	WANG Xiao-ping
	SUN Lin
	NI Qing
	SHAO Bing
	ZHANG Jing
	TIAN Xin

Cite this article:

WU Wei,WANG Xiao-ping,SUN Lin, et al. Relationship study between the expression and polymorphism of NLRP3 inflammatory bodies and the level of Caspase-1 and the degree of coronary lesions in patients with acute coronary syndrome[J]. 中国当代医药, 2021, 28(1): 71-74.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I1/71

[1]	Sinha SK，Goel A，Madaan A，et al.Prevalence of Metabolic Syndrome and Its Clinical and Angiographic Profile in Patients With Naive Acute Coronary Syndrome in North Indian Population[J].J Clin Med Res，2016，8（9）：667-673.
[2]	Tanveer S，Banu S，Jabir NR，et al.Clinical and angiographic correlation of high-sensitivity C-reactive protein with acute ST elevation myocardial infarction[J].Exp Ther Med，2016，12（6）：4089-4098.
[9]	程敏菊，魏庆民，程敏静，等.急性冠脉综合征患者Gensini评分与MACE 的关系[J].医学临床研究，2018，35（2）：268-270.
[10]	丁修冬，薛凤凤，沙立娜，等.sdLDL、FABP 在冠心病诊断中的应用及Gensini 评分的相关研究[J].标记免疫分析与临床，2018，25（9）：39-43.
[12]	吕洁，蒋晓山，张晶，等.NLRP3 炎症小体组分NLRP3 及CARD8 基因的遗传变异与缺血性脑卒中[J].脑与神经疾病杂志，2020，28（10）：645-649.
[3]	Veltman D，Laeremans T，Passante E，et al.Signal transduction analysis of the NLRP3-inflammasome pathway after cellular damage and its paracrine regulation[J].J Theor Biol，2017，21（415）：125-136.
[4]	Sandanger ，Gao E，Ranheim T，et al.NLRP3 inflammasome activation during myocardial ischemia reperfusion is cardioprotective[J].Biochem Biophys Res Commun，2016，469（4）：1012-1020.
[5]	Klen J，Gorvcar K，Janez A，et al.NLRP3 Inflammasome Polymorphism and Macrovascular Complications in Type 2 Diabetes Patients[J].J Diabetes Res，2015，2015（6）：616 747.
[6]	Kastbom A，Arlestig L，Rantapaa-Dahlqvist S.Genetic varia nts of the NLRP3 inflammasome are associated with stroke in patients with rheumatoid arthritis[J].J Rheumatol，2015，42（10）：1740-1745.
[7]	Amsterdam EA，Wenger NK，Brindis RG，et al.2014 AHA/ACC guideline for the management of patients with non-STelevation acute coronary syndromes：a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines[J].J Am Coll Cardiol，2014，64（24）：e139-e228.
[8]	中国医师协会急诊医师分会，国家卫健委能力建设与继续教育中心急诊学专家委员会，中国医疗保健国际交流促进会急诊急救分会.急性冠脉综合征急诊快速诊治指南（2019）[J].中华急诊医学杂志，2019，28（4）：421-428.
[11]	Robertson S，Martínez GJ，Payet CA，et al.Colchicine therapy in acute coronary syndrome patients acts on caspase-1 to suppress NLRP3 inflammasome monocyte activation[J].Clin Sci，2016，130（14）：1237-1246.
[13]	Hamzic-Mehmedbasic A.Inflammatory Cytokines as Risk Factors for Mortality After Acute Cardiac Events[J].Med Arch，2016，70（4）：252-255.
[14]	Li Q，Kuang Y，Qiu J，et al.The correlation between plasma tissue factor and interleukin 18 and their significance in patients with acute coronary syndrome[J].Cardiovasc Toxicol，2015，15（3）：276-282.
[15]	Kang HJ，Bae KY，Kim SW，et al.Relationship between interleukin-1β and depressive disorder after acute coronary syndrome[J].Prog Neuropsychopharmacol Biol Psychiatry，2017，4（72）：55-59.