胰腺癌组织中转化生长因子β1、上皮间质转化相关蛋白表达水平及其临床意义

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Abstract Objective To investigate the expression of transforming growth factor-β1 (TGF-β1) and epithelial mesenchymal transition (EMT) related proteins in pancreatic cancer tissue and its clinical significance.Methods A total of 60 patients with pancreatic cancer admitted to the Department of General Surgery of Guangzhou Twelfth People′s Hospital and Gastroenterology Department of the Second People′s Hospital of Guangdong Province from January 2019 to February 2020 were selected as research objects.The positive expression rates of TGF-β1 and EMT related proteins such as E-cadherin and Vimentin of pancreatic cancer tissue and paracancerous tissue of the patients were detected by immunohistochemistry method.The levels of serum carbohydrate antigen (CA) 199 and CA242 were measured.The levels of serum CA199 and CA242,lymph node metastasis and infiltration stage in patients with different expression levels of TGF-β1 and EMT related proteins in pancreatic cancer tissues were compared.And the relationship between the expression of TGF-β1 and EMT related proteins with the levels of serum CA199 and CA242,lymph node metastasis and infiltration stage in pancreatic cancer was analyzed.Results The positive expression rate of E-cadherin in pancreatic cancer tissue was lower than that in paracancerous tissue,while the positive expression rates of TGF-β1 and Vimentin in pancreatic cancer tissue were higher than those in paracancerous tissue,and the differences were statistically significant (P＜0.05).The levels of serum CA199 and CA242,the rate of lymph node metastasis and the proportion of infiltration stage as T3-T4 of patients with E-cadherin positive expression in pancreatic cancer tissue were lower than those of the patients with E-cadherin negative expression in pancreatic cancer tissue,and the differences were statistically significant (P＜0.05).The levels of serum CA199 and CA242,the rate of lymph node metastasis and the proportion of infiltration stage as T3-T4 of patients with TGF-β1 positive expression in pancreatic cancer tissue were higher than those of the patients with TGF-β1 negative expression in pancreatic cancer tissue,and the differences were statistically significant (P＜0.05).The levels of serum CA199 and CA242,the rate of lymph node metastasis and the proportion of infiltration stage as T3-T4 of patients with Vimentin positive expression in pancreatic cancer tissue were higher than those of the patients with Vimentin negative expression in pancreatic cancer tissue,and the differences were statistically significant (P＜0.05).The results of Kendall correlation analysis showed that the positive expression rate of TGF-β1 and Vimentin in pancreatic cancer tissue was positively correlated with serum CA199 and CA242 levels,lymph node metastasis rate and infiltration stage (P＜0.05),while the positive expression rate of E-cadherin in pancreatic cancer tissue was negatively correlated with serum CA199 and CA242 levels,lymph node metastasis rate and infiltration stage (P＜0.05).Conclusion In pancreatic cancer patients,the expression of TGF-β1 and Vimentin is up-regulated while the expression of E-cadherin is down regulated,which are related to tumor invasion and metastasis and may be reference indexes for tumor invasion and metastasis evaluation.

Key words： Pancreatic cancer Cancer tissue Transforming growth factor β1 Epithelial mesenchymal transition related protein Expression Invasion and metastasis

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