抗癌烷化剂三溴基丙酮酸对秀丽隐杆线虫衰老指标及寿命的影响

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Abstract Objective To explores the influence of different concentrations of 3-Bromopyruvate (3-BrPA)on the lifespan of Caenorhabditis elegans and tests of related aging indicators to systematically assess its impact on life conditions.Methods The same period of Caenorhabditis elegans was selected as the experimental object,3-BrPA of 3.125,6.250,12.500 and 25.000 mmol/L were selected as the experimental group,and 0.000 mmol/L were selected as the control group.A total of 135cases of Caenorhabditis elegans were selected for each concentration in the nematode life test group,and the survival curves of the each experimental group and the control group were recorded and plotted in the nematode swallowing capacity experiment,10 cases of Caenorhabditis elegans were selected in the each group,and the swallowing rate graph reflecting the aging degree of the Caenorhabditis elegans was recorded and plotted.Results Both Caenorhabditis elegans lifespan test and pharynx pumping ability test results showed that which in the experimental group of 3-BrPA of 3.125,25.000mmol/L were lower than those of the control group,and the differenceswere statistically significant(P＜0.05).There were no significant differences in the Caenorhabditis elegans lifespan test and pharynx pumping ability test results between the the experimental group of 3-BrPA of 6.250,12.500 mmol/L and the control group(P＞0.05).Conclusion 3-BrPA at 3.125and 25.000mmol/L showed a certain degree of damage to Caenorhabditis elegans;However,3-BrPA at the concentrations of 6.250 and 12.500mmol/L aren′t show significant toxic and side effect on Caenorhabditis elegans,and compared with the control group,the lifespan of the Caenorhabditis elegans is not shortened and prolonged.The occurrence of this special phenomenon may be related to the pyruvate concentration in the body of Caenorhabditis elegans and themechanism of dietary restriction (DR).The specific reasons are still being further studied.

Key words： Anticancer alkylating Agent 3-Bromopyruvate Caenorhabditis elegans Anti-aging Toxic side effect

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	Articles by authors
	WEI Qian-nan
	WANG Su-juan
	LIU Jia-qi
	BU Xiao-bo
	LI Dong-hang

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WEI Qian-nan,WANG Su-juan,LIU Jia-qi, et al. Influence of anticancer alkylating agent 3-Bromopyruvate on aging indexes and lifespan of Caenorhabditis elegans[J]. 中国当代医药, 2020, 27(17): 4-7.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2020/V27/I17/4

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