APE1基因单核苷酸多态性与肝细胞癌临床表型的相关性研究

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Abstract Objective To investigate the correlation between the single nucleotide polymorphisms of APE1 gene at the locus of -141T/G and 148Asp/Glu and different clinical phenotypes of hepatocellular carcinoma.Methods A total of 125 patients newly diagnosed with hepatocellular carcinoma who were hospitalized in the Interventional Radiology Department of the Affiliated Hospital of Nantong University from January 1 to December 31, 2018 were selected as the research objects to extract genomic DNA.The panel high-throughput data analysis was adopted to conduct APE1 genotyping and the correlation between each genotype and the clinical phenotype of hepatocellular carcinoma was analyzed.Results At locus -141, patients with heterozygous mutant genotype TG had an increased risk of later stage than patients with wild-type TT (OR=4.513, 95%CI：1.411-14.445, P＜0.05), the patients with homozygous mutant genotype GG had an increased risk of later stage than patients with wild-type TT (OR=4.444, 95%CI：1.217-16.233, P＜0.05),and the risk of later stage in patients with mutant genotype TG+GG was higher than that in patients with wild type TT (OR=4.491, 95%CI：1.457-13.848, P ＜0.05).There was no significant difference in the risk of stages of each genotype at locus 148 (P＞0.05).At locus -141,patients with heterozygous mutant genotype TG had an increased risk of portal vein invasion compared with patients with wild-type TT (OR=3.091, 95%CI：1.019-10.064, P＜0.05), the patients with homozygous mutant genotype GG had an increased risk of portal vein invasion compared with patients with wild-type TT (OR=3.789, 95%CI：1.025-14.009, P＜0.05), and the risk of portal vein invasion in patients who carried the mutant genotype TG+GG was higher than that in patients with wild type TT (OR=3.302, 95%CI：1.061-10.276, P＜0.05).There was no significant difference in the risk of portal vein invasion among all genotypes at locus 148 (P＞0.05).There was no significant difference in the risk of lymph node metastasis among genotypes at locus -141 (P＞0.05).At locus 148, patients with homozygous mutation GG had an increased risk of lymph node metastasis compared patients with wild-type TT (OR=3.500, 95%CI：1.018-12.03, P＜0.05).At locus -141, patients with homozygous mutation GG had an increased risk of distant metastasis compared with patients with wild-type TT (OR=5.667, 95%CI：1.074-29.891, P＜0.05).There was no significant difference in the risk of distant metastasis among all genotypes at locus 148 (P＞0.05).Conclusion The single nucleotide polymorphisms of APE1 gene are correlated with clinical phenotypes of hepatocellular carcinoma such as BCLC stage,portal vein invasion, lymph node metastasis and distant metastasis.

Key words： APE1 Hepatocellular carcinoma Single nucleotide polymorphism Clinical phenotype

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	Articles by authors
	LU Xiao-hua
	ZHU Xiao-qing
	YUAN Hong-xin
	CHU Yu-shan

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LU Xiao-hua,ZHU Xiao-qing,YUAN Hong-xin, et al. Study on the correlation between single nucleotide polymorphisms of APE1 gene and clinical phenotype of hepatocellular carcinoma[J]. 中国当代医药, 2020, 27(13): 13-17.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2020/V27/I13/13

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