肠易激综合征患者5-羟色胺及5-羟色胺2B受体变化的研究

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Abstract Objective To observe the changes of 5-hydroxytryptamine (5-HT) and 5-HT2B receptor in the patients with irritable bowel syndrome (IBS).And to explore the role of 5-HT and 5-HT2B receptor in the pathogenesis of IBS.Methods From January 2016 to may 2017, 48 patients with diarrhea type IBS (IBS-D) and constipation type IBS (IBSC) were selected from the Department of Gastroenterology, Dalian Friendship Hospital, including 26 cases of IBS-D patients (IBS-D group), 22 cases of IBS-C patients (IBS-C group).In addition, those undergoing colonoscopy for polyps or cancer surveillance with negative results were included in the control group (28 cases).The abdominal symptom scale was used to score the subjects, and the Plasma 5-HT and 5-HT2B receptor concentrations were detected by ELISA.Specimens were obtained from the ascending colon during the colonoscopy, and the expression level of 5-HT mRNA and 5-HT2B receptor mRNA in colonic mucosa were detected by reverse transcription PCR.The relationship between 5-HT and 5-HT2B receptor levels and abdominal symptoms were calculated.Results The scores of abdominal symptoms in IBS-D and IBS-C groups were higher than those in the control group, the differences were statistically significant (P＜0.05).The plasma concentrations of 5-HT and 5-HT2B receptor in IBS-D and IBS-C groups were higher than those in the control group, the differences were statistically significant (P＜0.05).The expression of 5-HT and 5-HT2B receptor mRNA in colonic mucosa of IBS-D and IBS-C group were higher than those of control group, the differences were statistically significant (P＜0.05).Spearman analysis showed that the expression of plasma 5-HT in IBS-D and IBS-C group were positively correlated with abdominal symptom score (r=0.82, 0.79；P＜0.05).The plasma 5-HT2B receptor expression in IBS-D and IBS-C groups were positively correlated with abdominal symptom scores (r=0.78, 0.76；P＜0.05).There was no correlation between the expression of 5-HT and 5-HT2B receptor and abdominal symptom score in the control group (P＞0.05).Conclusion T5-HT and 5-HT2B are significantly increased in IBS patients, and plasma 5-HT and 5-HT2B are positively correlated with the abdominal symptom score of IBS patients, suggesting that 5-HT and 5-HT2B receptors may be involved in the occurrence of abdominal pain in IBS patients.

Key words： Irritable bowel syndrome 5-hydroxytryptamine 5-hydroxytryptamine 2B receptor Colon tissues Abdominal pain

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	Articles by authors
	SUN Yong-hong
	ZHANG Ye
	WANG Yu
	WANG Fu-cheng
	LI Qing
	SUN Xiu-li
	ZHANG Jing

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SUN Yong-hong,ZHANG Ye,WANG Yu, et al. Changes study of 5-hydroxytryptamine and 5-hydroxytryptamine 2B receptor in the patient with irritable bowel syndrome[J]. 中国当代医药, 2020, 27(11): 16-19.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2020/V27/I11/16

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