γ干扰素、骨桥蛋白、白细胞介-18在肺结核患者血清与痰液上清液中的表达与临床意义

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Abstract Objective To explore the expression and clinical significance of interferon-γ (IFN-γ), osteopontin (OPN)and interleukin-18 (IL-18) in serum and sputum supernatant from patients with pulmonary tuberculosis.Methods Serum and sputum samples were collected both from 89 patients with pulmonary tuberculosis (pulmonary tuberculosis group) and 30 healthy individuals (healthy control group) in our hospital from November 2016 to December 2018.The levels of the three cytokines mentioned above were respectively detected by double antibody sandwich enzyme linked immunosorbent assay (ELISA) technique and then the levels of IFN-γ, OPN, and IL-18 between the two groups were compared.Results The levels of IFN-γ, OPN, and IL-18 in the serum and sputum supernatant of the pulmonary tuberculosis group were higher than those of the healthy control group, and the differences were statistically significant (P＜0.05).Conclusion It is of great significance for the auxiliary clinical diagnosis and the judgment of the disease prognosis due to the elevation of the IFN-γ, OPN, and IL-18 in serum and sputum supernatants from patients with pulmonary tuberculosis.

Key words： Pulmonary tuberculosis；Interferon-&gamma , Osteopontin；Interleukin-18

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	DU Wei-qin
	REN Jian-bin▲

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DU Wei-qin,REN Jian-bin▲. Expression and clinical significance of interferon-γ, osteopontin and interleukin-18 in serum and sputum supernatant from patients with pulmonary tuberculosis[J]. 中国当代医药, 2020, 27(11): 12-15.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2020/V27/I11/12

[1]	Eurosurveillance editorial team.WHO publishes global tuberculosis report 2013[J].Euro Surveill，2013，18（43）：1-306.
[7]	张培元.肺结核诊断和治疗指南[J].中华结核和呼吸杂志，2001，24（2）：70-74.
[8]	高惠，吴晓虹.活动性肺结核患者血清巨噬细胞移动抑制因子水平变化及预后关系研究[J].临床肺科杂志，2019，24（3）：527-531.
[2]	Ridruechai C，Sakurada S，Yanai H，et al.Association between circulating full-length osteopontin and IFN-gamma with disease status of tuberculosis and response to successful treatment[J].Southeast Asian J Trop Med Public Health，2011，42（4）：876-889.
[3]	Zhu YA，Jia HY，Chen JN，et al.Decreased osteopontin expression as a peliable prognostic indicator of improvement in pulmonary tuberculosis：impact of the level of interferongamma-inducible protein 10[J].Cell Physiol Biochem，2015，37（5）：1983-1996.
[4]	Inomata S，Shijubo N，Kon S，et al.Circulating interleukin-18 and osteopontin are useful to evaluate disease activity in patients with tuberculosis[J].Cytokine，2005，30（4）：203-211.
[5]	Chowdhury IH，Choudhuri S，Sen A，et al.Serum interleukin 6 （IL-6） as a potential biomarker of disease progression in active pulmonary tuberculosis following anti-tuberculosis drug therapy[J].Mol Immunol，2015，63（2）：601-602.
[6]	Domingo-Gonzalez R，Prince O，Cooper A，et al.Cytokines and chemokines in Mycobacterium tuberculosis infection[J].Microbiol Spectr，2016，4（5）：1-58.
[9]	蒋纾芳，张嵘，冯晓燕.细胞因子和microRNA 与结核分枝杆菌感染的研究进展[J].生物技术通讯，2014，25（6）：884-888.
[10]	Belay M，Legesse M，Mihret A，et al.Lipoarabinomannanspecific TNF-α and IFN-γ as markers of protective immunity against tuberculosis：a cohort study in an endemic setting[J].APMIS，2015，123（10）：851-857.
[11]	张雅婷.OPN、IL-18、IFN-γ 及hs-CRP 在肺结核者中的检测意义[J].国际检验医学杂志，2018，39（5）：632-634.
[12]	冯佩璐，袁岚.血清IFN-γ、OPN、IL-18 检测对肺结核患者的临床意义[J].检验医学与临床，2017，14（21）：3168-3169，3172.
[13]	袁媛.活动性肺结核患者抗结核治疗后血清炎症因子动态水平改变与预后关系研究[J].国际检验医学杂志，2018，39（3）：327-329.
[14]	郭淑湘，马冬梅，刘慧芳，等.肺结核患者血清干扰素-γ、白细胞介素-18 和骨桥蛋白水平的动态变化及临床意义[J].实用医学杂志，2013，29（4）：562-564.
[15]	袁媛，陈骥.IL-18、OPN、INF-γ 及hs-CRP 在肺结核中的检测意义[J].湖南师范大学学报（医学版），2016，13（2）：117-120.
[16]	Millington KA，Innes JA，Hackforth S，et al.Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load[J].J Immunol，2007，178（8）：5217-5226.
[17]	方毅敏，马志明，何桥，等.肺结核病灶组织中γ-干扰素的表达及其临床意义[J].实用医学杂志，2010，26（13）：2328-2330.
[18]	Wang KX，Denhardt DT.Osteopontin：role in immune regulation and stress responses[J].Cytokine Growth Factor Rev，2008，19（5-6）：333-345.
[19]	Koguchi Y，KawakamiK，UezuK，et al.High plasma osteopontin level and its relationship with interleukin-12-mediated type 1 T helper cell response in tuberculosis[J].Am J Respir Crit Care Med，2003，167（10）：1355-1359.
[20]	Ashkar S，Weber GF，Panoutsakopoulou V，et al.Eta-1（osteopontin）：an early component of type-1 （cell-mediated）immunity[J].Science，2000，287（5454）：860-864.
[21]	乔以凤.肺结核患者治疗前后血清OPN、IL-2 和VEGF水平的变化及临床意义[J].放射免疫学杂志，2013，26（6）：729-730.
[22]	马志明，肖芃，谭俊豪，等.骨桥蛋白在肺结核组织中的表达及其临床意义[J].广东医学，2010，31（5）：572-574.
[23]	Munk RB，Sugiyama K，Ghosh P，et al.Antigen-independent IFN-γ production by human naive CD4 T cells activated by IL-12 plus IL-18[J].PLoS One，2011，6（5）：e18553.
[24]	Kinjo Y，Kawakami K，Uezu K，et al.Contribution of IL-18 to Th1 response and host defense against infection by Mycobacterium tuberculosis：a comparative study with IL-12p40[J].J Immunol，2002，169（1）：323-329.