辅酶Q10脂质体的制备及其抗紫外线损伤的作用

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Abstract Objective To study the preparation of coenzyme Q10 liposome and its anti-ultraviolet damage effect.Methods The coenzyme Q10 liposomes were prepared by injecting ethanol,then animal experiments were performed.A total of 32 SPFmice were randomly divided into normal(CON)group,model(MOL)group,coenzyme Q10 liposome(COQ)group,and titanium dioxide(TiO2)group,8mice in each group.Themice in the CON group were not subjected to any treatment after depilation,and the remaining groups ofmice were smeared with the corresponding cream and irradiated with ultraviolet rays every day after depilation.Among them,the MOL group was smeared with the blank cream,the COQ group was smeared with the coenzyme Q10 liposome,and the TiO2 group was smeared with the TiO2 cream.The irradiation dose was theminimum amount of erythema,and the experiment lasted 8 weeks.After the end of the experiment,the skin pathology and the contents of malondialdehyde(MDA),superoxide dismutase(SOD),and matrix metalloproteinase(MMP-1),glutathione peroxidase(GSH-Px)in themice were determined.Results The optimum formulation of coenzyme Q10 liposome showed as the temperature was 60°C,the water phase of pH was 7.7 and other was 0.9%of coenzyme Q10 and 1.6%of lecithin.The encapsulation efficiency of coenzyme Q10 liposome was 44.7%,and the liposome was uniform in size and globular.The MDA content and the relative expression of MMP-1 in skin tissue of the mice in the MOL group were higher than those in the CON group,and the SOD contentwas lower than that in the CON group,with statistically significant differences(P＜0.05).The SOD content in the skin tissue of themice in the COQ group was higher than that in the MOL group,and the relative expression of MMP-1 was lower than that in the MOL group,the differences were statistically significant(P＜0.05).The MDA content in the skin tissue of themice in the TiO2 group was lower than that in the MOL group,and the difference was statistically significant(P＜0.05).The GSH-Px content in the skin tissue ofmice in the COQ group was higher than that in other groups,and the differences were statistically significant(P＜0.05).Conclusion The prepared coenzyme Q10 liposome is uniform in size and has anti-ultraviolet damage effect onmice skin.

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	WU Hai-you
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WU Hai-you,WU Yi,WU Tie. Preparation of coenzyme Q10 liposome and its anti-ultraviolet damage effect[J]. 中国当代医药, 2020, 27(9): 4-.

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[1]	Acosta MJ，Vazquez Fonseca L，Desbats MA，et al.Coenzyme Q biosynthesis in health and disease[J].Biochim Biophys Acta，2016，1857（8）：1079-1085.
[2]	张琰，宋金玉.辅酶Q10的应用研究进展[J].山西职工医学院学报，2017，27（3）：57-59.
[3]	邱楚群，梁美婷，江文菁，等.辅酶Q10联合Q伴侣对模型大鼠皮肤氧化损伤的改善作用[J].中国药业，2019，28（10）：9-12.
[4]	吴海游，梁美婷，邱楚群，等.三种含辅酶Q10防晒剂抗小鼠皮肤紫外线损伤的效果评价[J].中国皮肤性病学杂志，2017，31（4）：365-368.
[5]	Dragicevic N，Krajisnik D Milic J，et al.Development of hydrophilic gels containing coenzyme Q10-loaded liposomes：characterization，stability and rheologymeasurements[J].Drug Dev Ind Pharm，2019，45（1）：43-54.
[6]	Laredj LN，Licitra F，Puccio HM.The molecular genetics of coenzyme Q biosynthesis in health and disease[J].Biochimie，2014，100（5）：78-87.
[7]	李福民，刘媛，廖金凤，等.维生素E脂质体对小鼠皮肤光损伤的修复作用[J].中国临床药理学杂志，2019，35（5）：465-467.
[8]	赵国栋，夏强.辅酶Q10硫辛酸复配脂质体的制备与表征[J].食品与药品，2014，16（3）：153-158.
[9]	陈静怡，张莉，姬文捷，等.脂质体镶囊的研究进展[J].中国药学杂志，2017，52（3）：173-179.
[10]	Ding R，Li Z，Wang J，et al.Design and synthesis of galactose-biotin lipid materials for liposomes to promote the hepatoma cell-targetingeffect[J].JPharm Sci，2019，108（9）：3074-3081.
[11]	袁雍，黄萍，杨秀丽.脂质体经皮局部给药研究进展[J].中国药师，2014，17（7）：1227-1231.
[12]	彭浩，乔艳明，陈文强，等.用L9（34）正交实验筛选富硒香菇液体培养基[J].北方园艺，2014，（15）：152-157.
[13]	Benson HAE.Liposomes for topical and transdermal drug delivery[J].Curr Drug Deliv，2009，6（3）：217-226.
[14]	Li ZH，Li YY，Hou M，et al.Topically applied hypericin exhibits skin penetrability on nudemice[J].Lasers Med Sci，2018，33（6）：1279-1286.
[15]	Shim J，Kim MJ，Kim HK，et al.Morphological effect of lipid carriers on permeation of lidocaine hydrochloride through lipid membranes[J].Int JPharm，2010，388（1-2）：251-256.
[16]	Rattanapak T，Young K，Rades T，et al.Comparative study of liposomes，transfersomes，ethosomes and cubosomes for transcutaneous immunisation：characterisation and in vitro skin penetration[J].JPharm Pharmacol，2012，64（11）：1560-1569.
[17]	曹玉音，陈迎玉，王晖，等.辅酶Q10在活性氧相关疾病中应用研究进展[J].实用医学杂志，2018，34（20）：3482-3485.
[18]	林芳，马良娟.金属蛋白酶在皮肤光老化信号通路中的研究进展[J].临床与病理杂志，2018，38（11）：2502-2507.
[20]	吴文伯，陈敏亮，吴焱秋，等.富血小板血浆（PRP）对大鼠光老化皮肤胶原蛋白、TGF-β及MMP-1表达的影响[J].中国美容医学，2019，28（4）：64-67.
[19]	Lee HJ，Hwang E，Park B，et al.Methanol extract of bitter melon alleviates UVB-induced MMPs expression via MAP kinase and AP-1 signaling in human dermal fibroblasts in vitro[J].Phytother Res，2016，30（9）：1519-1526.