环孢霉素A对小鼠胚胎干细胞向心肌细胞分化的影响及调控机制

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Abstract Objective To explore the influence of Cyclosporine-A (CSA) on the differentiation of mouse embryonic stem cells (ESCs) into cardiomyocytes and its mechanism of regulation.Methods The differentiation system of mouse embryonic stem cells into cardiomyocytes by stages was used,and ESCs spontaneous group and CSA induction group were designed.The differentiation rate and count of cardiomyocytes were evaluated by cell immunohistochemistry and flow cytometry (FACS).The expression of CSA-induced cardiomyocyte markers was detected by cell immunohistochemistry.CSA was added at different stages of ES cell differentiation to observe whether CSA specifically acted on a particular differentiation phase.Another immunosuppressive agent including tacrolimus (calcineurin inhibitor:FK506) or 11R-VIVIT (nuclear factor of activated T cells:NFAT) was separately added in order to compare the differentiation rate of cardiomyocytes among the groups.Results In the ESCs spontaneous group,the differentiation rate of cardiomyocytes was (6.00±0.05)%,and the rate in the CSA-induction group was (60.00±0.08)%,which was displayed a statistical difference (P<0.05).The cardiomyocytes generated from induction possessed the unique characteristics.CSA specifically acted on mesodermal stem cells and promoted the differentiation of these cells into cardiac stem cells.The amount of myocardial cells was increased differentiated from mesodermal stem cells strongly promoted by CSA,while the number into epithelial cells was in decrease.After adding FK506 and 11R-VIVIT,there was no similar effect of CSA on promotion of cardiomyocyte differentiation.Conclusion Cyclosporine-A specifically acts on mesodermal stem cells and can powerfully induce these ESCs into the differentiated cardiomyocytes via non-NFAT pathway.

Key words： Cyclosporine A Cardiac stem cells Embryonic stem cells Cardiomyocytes Differentiation

Received: 08 February 2017

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R332

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	YAN Pei-shi
	DUAN Li-qin
	BI Sha-sha
	WANG Xiao-xu

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YAN Pei-shi,DUAN Li-qin,BI Sha-sha, et al. Influence of Cyclosporine A on the differentiation of mouse embryonic stem cells into cardiomyocytes and its regulatory mechanism[J]. 中国当代医药, 2017, 24(25): 4-4.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I25/4

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