神经生长因子及其低亲和力受体诱导肝星状细胞凋亡的初步研究

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Abstract Objective To observe the effect of nerve growth factor(NGF）and its low affinity receptor(p75 neurotrophin receptor,p75NTR)on the apoptosis and proliferation of rat hepatic stellate cells (HSC),and to explore the possible mechanism of apoptosis.Methods Rat hepatic stellate cells(HSC-T6)were incubated in vitro,HSC-T6 was incubated with NGF and NGF＋p75NTR with different concentrations.The cells were divided into the control group,the NGF group and the NGF＋p75NTR group.The control group was cultured with 0 ng/ml NGF＋0 ng/ml p75NTR,the NGF group was cultured with 100 ng/ml NGF,and the NGF＋p75NTR group was cultured with 100 ng/ml NGF＋100 ng/ml p75NTR.The flow cytometry was used to detect the apoptosis condition.The effect of HSC on the proliferation of NGF and p75NTR was observed by XTT,the expression of P53 and Caspase-3 were detected by immunohistochemical staining.Results The inhibitory effect of the NGF group on the proliferation of HSC was statistically significant(P＜0.05)with the NGF＋p75NTR group and the control group (P＜0.05).The flow cytometry results showed that the apoptosis rate of the control group was lower than that of the NGF group and the NGF＋p75NTR group(P＜0.05),the apoptosis rate of the NGF＋p75NTR group was higher than the NGF group(P＜0.05).The expression of P53 and Caspase-3 in the NGF group and the NGF＋p75NTR group were significantly higher than that in the control group (P＜0.05),the expressions in the NGF＋p75NTR group were significantly higher than that in the NGF group(P＜0.05).Conclusion NGF may inhibit HSC-T6 proliferation and promote its apoptosis through p75NTR,which may be related to the regulation of P53 and Caspase-3 expression.

Key words： Hepatic stellate cells Apoptosis Nerve growth factor Nerve growth factor low affinity receptor

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	Articles by authors
	ZHU Hai-yan
	WU Xiong-jian
	ZENG Bin
	XIE Zhi-jun

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ZHU Hai-yan,WU Xiong-jian,ZENG Bin, et al. The preliminary study on the apoptosis of hepatic stellate cells induced by nerve growth factor and its low affinity receptor[J]. 中国当代医药, 2018, 25(29): 8-10转14.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I29/8

[2]	张孝盈，宓余强.肝纤维化影响因素的研究进展[J].医学综述，2018，24（6）：1062-1066.
[1]	Kitano M，Bloomston PM.Hepatic stellate cells and mi-croRNAs in pathogenesis of liver fibrosis[J].J Clin Med，2016，5（3）：38.
[3]	AmorasEDSG，GomesSTM，FreitasFB，et al.NGF and P75NTR gene expression is associated with the hepatic fibrosis stage due to viral and nonViral causes[J].PLoS One，2015，10（3）：1-11.
[4]	Saxena NK，Anania FA.Adipocytokines and hepatic fibrosis[J].Trends Endoefinol Metab，2015，26（3）：153-161.
[5]	刘婷，徐铭益.肝纤维化的血清学诊断研究进展[J].实用肝脏病杂志，2016，19（1）：125-128.
[6]	Marrone G，Shah VH，Gracia-Sancho J.Sinusoidal communication in liver fibrosis and regeneration[J].J Hepatol，2016，65（3）：608-617.
[7]	Koyama Y，Xu J，Liu X，et al.New developments on the treatment of liver fibrosis[J].Dig Dis，2016，34（5）：589-596.
[8]	李妍，陆伦根.肝纤维化的分子基础与临床诊治现状[J].临床肝胆病杂志.2018，1（1）：12-15.
[9]	秦雨萌，陈胜华.肝纤维化进程中细胞外基质对肝星状细胞的作用及机制研究进展[J].实用肝脏病杂志，2017，20（3）：381-384.
[10]	曹留霞，张静，刘晓萍，等.NF-κB和 Ubc9在 HGF和 β-NGF诱导骨髓基质干细胞分化为肝细胞中的作用[J].青岛大学医学院学报，2016，52（4）：392-395.
[11]	张伟，刘翠芸，刘佩佩，等.神经生长因子对四氯化碳诱导的小鼠肝纤维化的影响[J].重庆医学，2015，44（15）：2024-2027.
[12]	Majdan M，Lachance C，Gloster A，et al.Transgenic mice expressing the intracellular domain of the p75 neurotrophin receptor undergo neuronal apoptosis[J].J Neurosci，1997，17（1）：6988-6998.
[13]	蔡凤桃，张学荣，孙林，等.眼镜蛇毒NGF通过PI3K/Akt促人肝星状细胞凋亡的机制研究[J].中国药理学通报，2018，34（1）：23-27.
[14]	张可星，张学荣，侯瑞雪，等.眼镜蛇毒NGF通过PI3K/AKT信号通路对肝星状细胞的影响[J].基因组学与应用生物学，2016，35（5）：1031-1305.
[15]	孙黔云.眼镜蛇毒因子在生命科学中的应用[J].生命科学，2016，28（1）：22-26.
[16]	Chen CH，Chen MF，Huang SJ，et al.Saikosaponin A induces apoptosis through mitochondria-dependent pathway in hepatic stellate cells[J].Am J Chin Med，2017，45（1）：351-368.
[17]	Valery K，Monica Y.Senescence of activated stellate cells limits liver fibrosis[J].Cell，2008，134（1）：657-667.
[18]	何雅军，朱海燕，舒建昌，等.神经生长因子抑制肝星状细胞增殖的观察[J].中华肝脏病杂志，2012，20（12）：912-914.
[19]	舒建昌，朱海燕，吕霞，等.神经生长因子对肝星状细胞胶原分泌及形态学的影响[J].中国病理生理杂志，2011，27（12）：2396-2398.
[20]	舒建昌，邓延梅，朱海燕，等.神经生长因子诱导肝星状细胞凋亡及其相关机制的研究[J].中国医师杂志，2012，14（2）：151-154.