cTfh细胞及相关miR-146a在乙肝疫苗记忆性免疫应答中的动态表达及关联研究

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Abstract Objective To detect the dynamic expression levels of miR-146a and circulating T follicular helper(cTfh)cell during immune response of hepatitis B vaccination,and then analyze the association between miR-146a,cTfh cell and the immune response of hepatitis B vaccination.Methods In July 2017,a group of healthy adults who had completed the standard procedure for the hepatitis B vaccination in Community Health Service Center of Dalang Country were recruited to receive one dose of hepatitis B vaccination,and peripheral blood samples were collected from 12 volunteers at different time points after vaccination,ELISA method was used to measure the concentration of Hepatitis B surface antibody(HBsAb)in serum;Flow cytometry was used to detect the proportion of Tfh cells,B cells,plasma cells,and memory B cells in peripheral blood mononuclear cells(PBMCs).Real-time fluorescence quantitative PCR was used to detect the relative expression of miR-146a.Results cTfh cell reached the highest level(P=0.001)on the 7thday after vaccination,and the HBsAb reached the highest level on the 14thday after vaccination,and the difference was statistically significant(P<0.001).There was a negative correlation between the Tfh cells and B cells(r=-0.426,P=0.03),and a positive correlation between the plasma cells and HBsAb (r=0.330,P=0.04).There was no significant correlation between the expression level of miR-146a,cTfh cell and HBsAb (P＞0.05).Conclusion Tfh cells might play a role in the immune response to hepatitis B vaccination,but not demonstrate the association between miR-146a and the immune response of hepatitis B vaccination.

Key words： miR-146a T follicular helper cell Hepatitis B vaccination Immune response Association

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	Articles by authors
	YU Zu-wei
	LI Yu-lian
	CHEN Li-hua
	YIN Ming-juan
	HUANG Ling-feng
	ZHANG Yan

Cite this article:

YU Zu-wei,LI Yu-lian,CHEN Li-hua, et al. Expression dynamics and association of cTfh cells and miR-146a in the immune response to hepatitis B vaccination[J]. 中国当代医药, 2018, 25(20): 12-15.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I20/12

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