中药牛大力对尿酸盐诱导小鼠模型滑膜细胞炎症的影响

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Abstract Objective To investigate the influence of Chinese herbal medicine Millettia speciosa champ on the synovial cells inflammation of mouse model induced by urate.Methods A total of 60 male Wistar mice of grade SPF were randomly divided into the normal control group,the model group,and the study group(low doses group of 4.55 g/kg,middle does group of 9.10 g/kg,and high doses group of 13.65 g/kg),10 cases in each group.Except for the normal control group,the drug was continuously given to 7 d,while the normal control group and the model group did not take any medicine,and given the same amount of normal saline daily to the stomach,the other four groups were injected urate into the right ankle joint to create an acute gouty arthritis model.The levels of serum nitric oxide(NO),interleukin-1β(IL-1β),tumor necrosis factor(TNF-α),prostaglandin E2(PGE2),and serum uric acid(UA)were measured and compared between before model establishment and after injection of urate 48 h.After successful examination,the model group was sacrificed and the joint capsule and synovial tissue were selected.HE staining was used to observe the pathological changes of the synovial tissue of the right ankle joint in mice.Each dose group was decocted with low,medium and high doses of Millettia speciosa champ,each group was treated with gavage for 1 times at 9 am,for 7 consecutive days.In the treatment of 3 and 7 d,the levels of serum inflammatory factors and serum UA levels in the low,medium and high doses groups of Millettia speciosa champ were measured again,and after 7 d treatment,the synovial tissues of two groups were stained with HE to observe the pathological changes of synovial tissue in the right ankle.Results The level of serum NO in four groups after injection of urate 48 h was lower than that before modeling,while the serum levels of IL-1β,TNF-α,PGE2and serum UA were higher than those before the modeling,with significant difference (P＜0.05).HE staining showed that there was congestion and swelling in the blood vessels of synovial tissue in the medium and high doses groups of Millettia speciosa champ,and the infiltration of inflammatory cells was obvious.The serum NO levels in the low,medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were significantly higher than those in the model group,and the level of serum inflammatory factors and serum UA in the low,medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were lower than that in the model group,with significant difference (P＜0.05).The serum NO levels in the low,medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were significantly higher than those before treatment,and the level of serum inflammatory factors and serum UA in the low,medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were lower than that before treatment,with significant difference (P＜0.05).After treatment for 7 d,HE staining showed that the inflammatory infiltration of the synovial tissue in the low,middle and high doses group of Millettia speciosa champ was improved and the disease was relieved,but there was no obvious improvement in the synovial tissue inflammation in the model group.Conclusion Chinese herbal medicine Millettia speciosa champ can help alleviate the inflammation of the mouse model synoviocytes induced by urate,which is worthy of popularizing.

Key words： Millettia speciosa champ Urate Mice Synovial cells Inflammation

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	HUANG Hui
	CHEN Hong
	HUANG Gui-qiong
	YUAN Wei-wei

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HUANG Hui,CHEN Hong,HUANG Gui-qiong, et al. Influence of Chinese herbal medicine Millettia speciosa champ on the synovial cells inflammation of mouse model induced by urate[J]. 中国当代医药, 2018, 25(20): 8-11.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I20/8

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