猪冠状动脉微栓塞后心肌细胞凋亡与内质网应激相关蛋白CHOP表达的关系

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Abstract Objective To investigate the relation of myocardial apoptosis and endoplasmic reticulum stress-related(CME)protein C/EBP homologous protein (CHOP)in swine following coronary microembolization.Methods Fifty swine were selected and randomly divided into the Sham group (n=25)and the CME group (n=25).Five subgroups were assigned according to time points(3,6,12,24,48 h),5 cases in each group.The CME model was established through injecting microspheres into the coronary artery,while swine in the Sham group received the same dose of normal saline instead.Cardiac function was measured with echocardiography.Histopathological changes were detected with HE.Microinfarction and apoptotic index were detected with hematoxylinbasic fuchsinpicric acid (HBFP)and TUNEL stainings,respectively.The expressions of CHOP and Caspase-3 proteins were determined with Western blot.Results Compared with the Sham groups,the cardiac function was decreased in varying degrees in the corresponding CME groups(P＜0.05).The cardiac function of 12 h following CME was the worst.Microinfarction rather than massive necrosis was found each time points following CME,mainly located in left ventricle and subendocardial myocardium.The difference,however,was not statistically significant(P＞0.05).Compared with the Sham groups,the apoptotic index in the corresponding CME groups were increased in varying degrees(P＜0.05).It peaked 12 h following CME.Apoptotic cardiomyocytes were mainly located in microinfarct area and its border area.Compared with the Sham groups,CHOP protein expression in the corresponding groups following CME enhanced in varying degrees(P＜0.05),showing dynamic changes.It peaked 12 h following CME.Conclusion CME induces cardiomyocyte apoptosis and cardiac dysfunction,in which enhanced CHOP and caspase-3 expression may be involved.

Key words： Coronary microembolization Apoptosis CHOP Endoplasmic reticulum stress

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	LIN Chong-qiang
	LIU Tao
	TANG Zhong-li
	LIU Yang-chun
	LI Lang

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LIN Chong-qiang,LIU Tao,TANG Zhong-li, et al. The relationship of myocardial apoptosis and endoplasmic reticulum stressrelated protein CHOP in swine following coronary microembolization[J]. 中国当代医药, 2018, 25(12): 9-13.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I12/9

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