丙泊酚对脂多糖诱导的人脐静脉内皮细胞p38 MAPK/NF-κB信号通路活性的影响

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Abstract bjective To study the influence of Propofol on the activity of p38,MAPK/NF-κ B signal pathway in human umbilical vein endothelial cells(HUBECs)induced by lipopolysaccharide (LPS).Methods HUBECs cultured in vitro were randomly divided into the 4 groups:control group(group C)was given Intralipid 20 mg/L,LPS group(L group)was given LPS 10 mg/L,Propofol group (P group)was given Propofol 20 mg/L,Propofol+LPS group (PL group)was given Propofol 20 mg/L and LPS 10 mg/L.The change in the content of p38 MAPK,p-p38 MAPK and NF-κB were detected at five time points of 1,2,3,6,and 12 h.Results When administered 1 h,the gray value of p-p38 MAPK and NF-κB in group L was 10.32±0.73,60.32±5.73 respectively,which increased significantly compared with group C(P＜0.05).When administered 2 h,the gray value of p-p38 MAPK and NF-κB in group L was 9.23 ±0.53,69.23±5.53,which increased significantly compared with group C (P＜0.05),and the gray value of PL group was 6.32±0.35,which was significantly lower than that of group L.Administration of 3 h,p-p38 MAPK,NF-κB gray value in group L was 11.13±0.23,71.13±5.23 respectively,which increased significantly compared with group C(P＜0.05).p-p38 MAPK,NF-κB gray value was 8.95±0.21,56.95±5.21 respectively in group PL,which decreased significantly compared with group L(P＜0.05).Administration of 6 h,p-p38 MAPK,NF-κB gray value was 12.16±0.20,73.16±4.20 respectively in group L,which increased significantly compared with group C (P＜0.05).p-p38 MAPK,NF-κB gray value was 9.65±0.32,58.65±4.32 respectively in group PL,which decreased significantly compared with the L group (P＜0.05).Administration of 12 h,p-p38 MAPK,NF-κB gray value was 18.19±0.31,91.19±6.31 respectively,which increased significantly compared with group C (P＜0.05).p-p38 MAPK,NF-κB gray value in group PL was 12.65±0.12,63.36±5.12 respectively,which decreased significantly compared with group L(P＜0.05).Conclusion LPS stimulating HUBECs can increase the expression of p-p38 MAPK and NF-κB,and inhibit this increase after Propofol injection may be one of the mechanisms of Propofol anti-inflammation.

Key words： Propofol Human umbilical vein endothelial cells Inflammation

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	WANG Xin
	LEI Xiao-wen
	CHEN Dan
	LIU Bin

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WANG Xin,LEI Xiao-wen,CHEN Dan, et al. The effect of Propofol on the activation of p38 MAPK/NF-κB signal pathway in human umbilical vein endothelial cells induced by lipopolysaccharide[J]. 中国当代医药, 2018, 25(9): 4-6转11.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I9/4

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