间歇性低氧在大鼠心肌缺血/再灌注损伤中的保护机制

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Abstract Objective To study the expression of phosphatidylinositide 3-kinase signaling pathway(PI3K),hypoxia-inducible factor 1α (HIF-1α),vascular endothelial growth Factor-2(VEGF-2)and to studied the correlation protection mechanism of intermittent hypoxia on heart ischemia/reperfusion(I/R)injury in rats.Methods 72 rats were randomly divided into the sham-operated group(n=18),the myocardial I/R group(n=18),the myocardial I/R+intermittent hypoxia(IH)group(I/R+IH)(n=18)and the myocardial I/R+IH+phosphatidylinositide 3-kinase(PI3K)inhibitor(wortmannin)group(IH+I/R+PI3K inhibitor group)(n=18).Mice were exposed to cycles to establish the IH model.I/R model was established by ligating the left anterior descending coronary artery.The MDA and GSH of cardic muscle tissues,and the serum biomarkers CK-MB in the four groups were detected.The LV end-diastolic volume(LVEDV),LV end-systolic(LVESV),LV ejection fraction(LVEF)of the rats by echocardiography after 8 weeks were assessed.The expression of phosphorylation of protein kinase B (Akt),HIF-1α,VEGF-2 was analyzed by Westen-blot.Results The level of MDA,CK-MB,LVEDV,LVESV of the I/R group was higher than that of the sham-operated group,the value of GSH,LVEF and the expression of AKt,HIF-1α,VEGF-2 of the I/R group was lower than that of the sham-operated group,with significant difference (P＜0.05).The level of MDA,CK-MB,LVEDV,LVESV of the I/R+IH group was lower than that of the I/R group,the value of GSH,LVEF and the expression of AKt,HIF-1α,VEGF-2 of the I/R+IH group was higher than that of the I/R group,with significant difference(P＜0.05).The level of MDA,CK-MB,LVEDV,LVESV of the I/R+IH+PI3K inhibitor group was higher than that of the sham-operated group,the value of GSH,LVEF and the expression of AKt,HIF-1α,VEGF-2 of the I/R+IH+PI3K inhibitor group was lower than that of the sham-operated group,with significant difference(P＜0.05).Conclusion The PI3K/Akt pathway and the expression of HIF-1α,VEGF-2 is important for IH-induced cardioprotection and may represent aviable target to reduce myocardial I/R injury.

Key words： Intermittent hypoxia Phosphatidylinositide 3-Kinase Myocardial ischemia reperfusion Hypoxia-inducible factor 1&alpha Vascular endothelial growth factor-2

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	WANG Yuan-yuan
	CAO Jian
	WAN Jian-hua

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WANG Yuan-yuan,CAO Jian,WAN Jian-hua. Protection mechanism of intermittent hypoxia on heart ischemia/reperfusion injury in rats[J]. 中国当代医药, 2018, 25(1): 4-7.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I1/4

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