褪黑素通过H IF-1α/VEGF信号通路减轻急性肾损伤的研究

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Abstract Objective To investigate the expression and influence ofmelatonin on hypoxia-inducible factor（HIF-1α）and vascular endothelial growth factor（VEGF）in acute kidney injury induced by lipopolysaccharide（LPS）. Methods 30 Sprague-Dawly（3-5 weeks）ratswere selected and randomly divided into the control group（n=10），the LPS group（n= 10，500μg/kg LPSwas injected intravenously）and the Mel+LPSgroup（n=10，melatonin was administrated orally，15min later，LPSwas given），followed by 48 hours，the ratswere killed.The thiobarbituric acid（TBA）testwas given to detect the level of MDA and the activity of superoxide dismutase （SOD）.The mRNAs and proteins of HIF-1αand VEGF were determined by RT-qPCR and immunohistochemistry.Results There was renal tubular epithelial cells swelling，renal interstitial edema and inflammation in the LPS group，those symptoms was attenuant in Mel+LSP group.The level of Scr and BUN in the LPS group［（35.56±1.83）mmol/L and　（46.23±2.87）μmol/L］was higher than that in the control group　［（12.69±0.97）mmol/L and （16.73±1.26）μmol/L］and the Mel+LPSgroup［（23.17±1.33）mmol/L and（35.67±2.25）μmol/L］（P＜0.05）.The level of MDA in the LPSgroup ［（697.45±43.13）nmol/（mg·prot）］was higher than that in the control group　［（354.35±28.12）nmol/（mg· prot）］and the level of MDA in the Mel+LPS group　［（455.37±30.79）nmol/（mg·prot）］was lower than that in the LPS group　（P＜0.05）.The level of SOD in the LPS group　［（3.75±0.23）U/（mg·prot）］was lower than that in the control group［（2.93±0.29）U/（mg·prot）］and Mel+LPSgroup［（3.20±0.27）U/（mg·prot）］（P＜0.05）.The level of HIF-1αand VEGFmRNA and protein in the LPS group was higher than that in the control group，and less than that in the Mel+LPS group.Con-clusion By inhibiting the production of MDA and up regulating the activity of SOD，Melcan regulate the expression of VEGF and HIF-1α，and plays a role in protecting LPS induced AKI renal function.

Key words： Melatonin；Renal injury hypoxia inducible factor-1&alpha ；Vascular endothelial growth factor

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	FENG Jie
	TONG Xiao-ya
	YU Jia-li
	PAN Zi-yu
	ZHA Yan

Cite this article:

FENG Jie,TONG Xiao-ya,YU Jia-li, et al. Study on melatonin alleviated acute kidney injury through the HIF-1α/ VEGF pathway[J]. 中国当代医药, 2016, 23(29): 12-15.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I29/12

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