不同处理因素对呼吸道合胞病毒感染人支气管上皮细胞表达血管内皮生长因子的影响

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Abstract Objective To explore the impact of different treatment factors on expression of vascular endothelial growth factor（VEGF）in primary cultured nornal human bronchial epithelial cells（NHBE）infected with respiratory syncytial virus（RSV）.Methods The impact of different treatment factors on expression NHBE VEGF infected with RSV was tested by using fluorescence quantitative PCR and ELISA.Results After NHBE infected with RSV，the expression of NHBE VEGFwas significantly increased，and with obvious time dependence and concentration dependence，Dexamethasone could significantly inhibit expression of VEGF in NHBE infected with RSV，and the difference was statistically significant compared with RSV infection group　（P＜0.05）.25 hydroxy vitamin D3alone using could stimulate that NHBE expressed VEGF，but it could inhibit that NHBE infected with RSV expressed VEGF，and the difference was statistically significant compared with RSV infection group　（P＜0.05）.Conclusion RSV increases the expression of VEGF in bronchial epithelium，while Dexamethasone and 25 hydroxy vitamin D3can reduce this effect.

Key words： Bronchial asthma Respiratory virus Vascular endothelial growth factor Bronchial epithelium Vitamin D Dexamethasone

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	Articles by authors
	GAO Yuan-mei
	XU Zhong
	HUANG Dong-jian
	CAIShao-xi

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GAO Yuan-mei,XU Zhong,HUANG Dong-jian, et al. Im pact of different treatment factors on expression of vascular endothelial grow th factor in primary cultured human bronchial epithelial cells in-fected with respiratory virus[J]. 中国当代医药, 2016, 23(27): 15-18.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I27/15

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