关节腔注射用雷公藤甲素微球的制备与毒性评价

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Abstract Objective To evaluate the quality and toxicity of triptolide microspheres for intra-articular injection.Methods Triptolide microspheres was prepared by emulsion solvent evaporation method.Surface morphology and size of microspheres were observed by transmission electron microscope.The formation of microspheres was investigated with infrared spectrometer.Entrapment efficiency and drug loading was determined by HPLC.The release of microspheres in vitro was fitted by mathematical model.The toxicity of microspheres was evaluated using hematological parameters and biochemical indexes.Results The microspheres were spherical in shape with uniform size.The mean diameter,encapsulation efficiency and drug loading was 4.75 μm,(74.37±2.30)%and(1.53±0.27)%respectively.The release of microspheres was fit for Ritger-Peppas equation.There were no significant differences of blood test between microspheres and control group.Conclusion Triptolide microspheres might be used as a new preparation for intra-articular injection.

Key words： Triptolide Microspheres Toxicity Ⅰntra-articular injection

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	WANG Li-juan
	CHE Ke-ke
	ZHANG Ru-chao

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WANG Li-juan,CHE Ke-ke,ZHANG Ru-chao. Preparation and toxicity evaluation of triptolide microspheres for intraarticular injection[J]. 中国当代医药, 2016, 23(30): 4-7转12.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I30/4

[1]	Horisawa E，Hirota T，Kawazoe S，et al.Prolonged anti-inflammatory action of DL-lactide/glycolide copolymer nanospheres containing betamethasone sodium phosphate for an intraarticular delivery system in antigen-induced arthritic rabbit [J].Pharm Res，2002，19（4）：403-410.
[2]	Horisawa E，Kubota K，TuboiⅠ，et al.Size-dependency of DL-lactide/glycolide copolymer particulates for intra-articular delivery system on phagocytosis in rat synovium[J].Pharm Res，2002，19（2）：132-139.
[3]	Fernández-Carballido A，Herrero-Vanrell R，Molina-MartínezⅠT，et al.Biodergradable ibuprofen-loaded PLGA microspheres for intraarticular administration.Effect of Labrafil addition on release in vitro[J].Ⅰnt J Pharm，2004，279（1-2）：33-41.
[4]	Li Z，Huang L.Sustained delivery and expression of plasmid DNA based on biodegradable polyester，poly（D，L-lactideco-4-hydroxy-L-proline）[J].J Contr Rel，2004，98（3）：437-446.
[5]	Javier RV，Ⅰrene BO，Rocío HV，et al.Optimising the controlled release of dexamethasone from a new generation of PLGA-based microspheres intended for intravitreal administration[J].Eur J Pharm Sci，2016，92（9）：287-297.
[6]	Guo WJ，Quan P，Fang L.Sustained release donepezil loaded PLGA microspheres for injection：preparation，in vitro and in vivo study[J].Asian J Pharm Sci，2015，10（5）：405-414.
[7]	蒋朝军，杨清敏，胡筱菲，等.新型利培酮PLGA微球的制备及体外释放研究[J].中国药学杂志，2011，46（2）：124-127.[8]Mao S，Shi Y，Li L，et al.Effects of process and formulation parameters on characteristics and internal morphology of poly（d，l-lactide-co-glycolide）microspheres formed by the solvent evaporation method[J].Eur J Pharm Biopharm，2008，68（2）：214-223.
[9]	Dhakar RC，Maurya SD，Aggarawal S.Design and evaluation of SRM microsphere of metformin hydrochloride[J].Pharmacie Globate，2010，1（7）：1-5.
[10]	Suhaida MG，YahyaⅠB，Darmawati MY.Preparation of naltrexone hydrochloride loaded poly（DL-lactide-co-glycolide）microspheres and the effect of polyvinyl alcohol（PVA）as surfactant on the characteristics of the microspheres[J]. Med J Malaysia，2004，59（Suppl B）：63-64.
[11]	刘明，董静，杨亚江，等.雷公藤甲素聚乳酸纳米粒载药体系的研究[J].中国药科大学学报，2004，35（2）：117-121.
[12]	McGinity JW，O′Donnell PB.Preparation of microspheres by the solvent technique[J].Adv Drug Deliv Rev，1997，28（1）：25-42.
[13]	O′Hagan DT，Jeffery H，Dams SS.The preparation and characterization of poly（lactide-co-glycolide）microparticles：Ⅲ. Microparticle/polymer degradation rates and the in vitro release of a model protein[J].Ⅰnt J Pharm，1994，103（1）：37-45.
[14]	Eliana L，Flavio F，Maria TB.Surface drug removal from ibuprofen-loaded PLA microspheres[J].Ⅰnt J Pharm，2000，196（1）：1-9.
[15]	Ritger PL，Peppas NA.A simple equation for description of solute releaseⅡ.Fickian and anomalous release from swellable devices[J].J Contr Rel，1987，5（1）：37-42.
[16]	冯群，孙蓉.雷公藤多苷片抗炎作用及伴随肝毒性研究[J].中药新药与临床药理，2014，25（6）：713-716.
[17]	邱颖文，吴松武，吴贤仁.雷公藤多苷的不良反应[J].西北药学杂志，2004，19（5）：220-222.
[18]	贾传春，王秀娟.雷公藤多苷片的临床不良反应[J].中国医院药学杂志，2000，20（5）：316-317.