孟鲁司特联合沙美特罗替卡松对支气管哮喘患者Th17细胞相关细胞因子表达的影响

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Abstract ObjectiveTo observe effect of oral Montelukast combined with Salmeterol Fluticasone inhalation on experssion levels of cytokines related to T helper(Th)17 cells transforming growth factor beta(TGF-beta),interleukin(IL)-17 and IL-23 in patients with new-onset bronchial asthma.MethodsAccording to different treatmentmethods，sixty patientwith new-onset bronchial asthma admitted into our hospital from October 2014 to April 2016 were divided into routine treatment group(n=20)and Montelukast combined with Salmeterol Fluticasone group whom received Montelukast combined with Salmeterol Fluticasone (n=40).Another 20 health people were selected as healthy group.The serum and sputum supernatan levels of inflammatory factors such as TGF-β,IL-17 and IL-23 were measured by ELISA before and after outpatient treatment for 3 months.ResultsCompared with healthy group,the serum and sputum supernatan levels of inflammatory factors such as TGF-β,IL-17 and IL-23 was higher that in the routine treatment group and the Montelukast combined with Salmeterol Fluticasone group,and the difference was statistically significant(P<0.01).After treatment for 3 months,the expression level TGF-βand IL-23 of two groups was significantly lower than that before treatment(P<0.05);compared with routine treatment group,expression levels of serum TGF-βand IL-23 in the Montelukast combined with Salmeterol Fluticasone group decreased significantly (P<0.05).There was no significant change in the expression of IL-17 in the serum and sputum supernatant of two groups before and after treatment for 3 months (P>0.05).ConclusionCytokines related cytokines Th17 cellsmay play important roles in the development of asthma,and using of Montelukast combined with Salmeterol Fluticasone can inhibit the experssion of cytokines related to Th17 in patientswith asthma.

Key words： Bronchial asthma Cytokines related to Th17 cells Montelukast Salmeterol Fluticasone

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	FENG Xiu-li
	DING Chang-jun

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FENG Xiu-li,DING Chang-jun. Effect of M ontelukast combined with Salmeterol Fluticasone on the expression of cytokines related to Th17 cells in patientswith bronchialasthma[J]. 中国当代医药, 2016, 23(33): 27-30.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I33/27

[13]	蒋鲲，陈和斌，王莹，等.IL-17和TGF-β1在支气管哮喘患儿血清及支气管肺泡灌洗液中的变化及临床意义[J].中国当代儿科杂志，2013，15（8）：604-608.
[1]	Cao Y，Sun Z，Liao L，et al.Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo[J].Biochem Biophys Res Commun，2005，332（2）：370-379.
[2]	Kim Y，Lee S，Kim YS，et al.Regulation of Th1/Th2 cells in asthma development：a mathematicalmodel[J].Math Biosci Eng，2013，10（4）：1095-1133.
[3]	Borish L，Aarons A，Rumbyrt J，et al.Interleukin-10 regulation in normal subjects and patientswith asthma[J].JAllergy Clin Immunol，1996，97（6）：1288-1296.
[4]	Dulek DE，Newcomb DC，Toki S，et al.STAT4 deficiency fails to induce lung Th2 or Th17 immunity following primary or secondary respiratory syncytial virus（RSV）challenge but enhances the lung RSV-specific CD8+T cell immuneresponse to secondary challenge[J].JVirol，2014，88（17）：9655-9672.
[5]	Harrington LE，Hatton RD，Mangan PR.Interleukin 17-producing CD4+effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages[J].Nat Immunol，2005，6（11）：1123-1132.
[6]	Durrant DM，Metzger DW.IL-12 can alleviate Th17-mediated allergic lung inflammation through induction of pulmonary IL-10 expression[J].Mucosal Immunol，2010，3（3）：301-311.
[7]	Gökmen MR，Dong R，Kanhere A，et al.Genome-wide regulatory analysis reveals that T-bet controls Th17 lineage differentiation through direct suppression of IRF4[J].J Immunol，2013，191（12）：5925-5932.
[8]	Moir LM，Burgess JK，Black JL.Transforming growth factor beta 1 increases fibronectin deposition through integrin receptor alpha 5 beta 1 on human airway smooth muscle[J].J Allergy Clin Immunol，2008，121（4）：1034-1039.
[9]	Secker GA，Shortt AJ，Sampson E，et al.TGF beta stimulated re-epithelialisation is regulated by CTGF and Ras/MEK/ ERK signaling[J].Exp Cell Res，2008，314（1）：131-142.
[10]	Wakashin H，Hirose K，Maezawa Y，et al.IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice[J].Am JRespir Criti Care Medicine，2008，178（10）：1023-1032.
[14]	覃毅华.支气管哮喘急性发作期患者白细胞介素（IL-17）及超敏C反应蛋白的水平变化及临床意义[J].中国现代医生，2014，52（18）：23-25.
[11]	Moraes-Vieira PM，Larocca RA，Bassi EJ，et al.Leptin deficiency impairs maturation of dendritic cells and enhances induction of regulatory T and Th17 cells[J].Eur J Immunol，2014，44（3）：794-806.
[12]	Hackett TL，Warner SM，Stefanowicz D，et al.Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1[J].Am JRespir Crit Care Med，2009，180（2）：122-133.
[15]	Durrant DM，Gaffen SL，Riesenfeld EP，et al.Development of allergen-induced airway inflammation in the absence of T-bet regulation is dependent on IL-17[J].J Immunol，2009，183（8）：5293-5300.
[16]	Lendermon EA，Dodd-o JM，Coon TA，et al.CD8（+）IL-17 （+）T cellsmediate neutrophilic airway obliteration in T-bet-deficient mouse lung allograft recipients[J].Am J Respir Cell Mol Biol，2015，52（5）：622-633.