大鼠脊髓源性少突胶质前体细胞体外机械拉伸损伤模型的建立与评价

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Abstract ObjectiveTo establish a model of stretch-induced mechanical injury on cultured oligodendrocyte precursor cells(OPCs)in vitro with the FX-4000TTMsystem,in order to further explore the role and mechanism of OPCs in spinal cord injury and repair.MethodsOPCs were separated from spinal cord of neonatal rats and then purified with the shaking method in vitro.Immunofluorescence assay was applied to identify the separated cells.A FX-4000TMsystem was used to apply cyclic stretch.The purified OPCs were randomly divided into four groups∶group A (control group),group B (5%tensile strain),group C (10%tensile strain),group D(15%tensile strain).After OPCs were strained for 2 h by the FX-4000TTMsystem,morphology of OPCs was observed by using inverted microscope,viability of OPCs was measured by MTT assay and apoptosis of the cells was detected by double staining flow cytometry,damage models were evaluated.ResultsThe percentage of purified OPCs in vitro was more than 90%.There were no significant differences between group A and group B in survival rate and cell morphology.Compared with group A,survival rate of OPCs in group C and D was significantly decreased(P＜0.05),apoptosis rate of OPCs was significantly increased(P＜0.05),and obvious pathological morphologic changes of OPCs were also observed in group C and D.However,there was a large loss of cell attachment and survival rate of OPCs was found to be less than 50%in group D.ConclusionThe model of stretch-induced mechanical injury on cultured OPCs can be established by applying 10%tensile strain with the FX-4000TTMsystem.

Key words： Oligodendrocyte precursor cells Cell culture In vitro model Mechanical strain Spinal cord injury

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	Articles by authors
	MA Xiang
	QI Yue-hong
	WEI Jian-feng
	LI Yan-ze
	BI Jing
	LYU Lei
	GUO Yong-qing

Cite this article:

MA Xiang,QI Yue-hong,WEI Jian-feng, et al. The establishment and evaluation of a model of stretch-induced mechanical injury on cultured oligodendrocyte precursor cells from neonatal rat spinal cord in vitro[J]. 中国当代医药, 2016, 23(36): 4-7.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I36/4

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