骨碎补总黄酮对骨质疏松症大鼠BMP-2蛋白表达及血清骨钙素水平的影响

Abstract
Figure/Table
References (20)
Related Citation (3)

Download: PDF (0 KB) HTML (48 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the effect of Total Flavonoids of RhizomaDrynariae on BMP-2 protein expression and serum bone gla protein level in rats with osteoporosis.Methods Osteoporosis model was established by removing rat ovary,and the rats were divided into normal group,sham operation group,model group,total Flavonoids of Rhizoma Drynariae group and Caltrate D Tablet group.In the seventh days after surgery,drugs were used once per day for 12 consecutive weeks.Morphological change of bone tissue in rats was observed by HE staining,and the expression of BMP-2 protein and the level of serum bone gla protein level in the femur of rats in each group was detected by ELISA.Results HE staining indicated that the number,density,distribution,and integrity of bone trabecula in the rats femoral bone tissue in the Total Flavonoids of Rhizoma Drynariae group were superior to those in the model group.The protein expression of BMP-2 in rats femoral bone tissue and the level of serum bone gla protein [(13.469±0.215)pg/ml and (1.61± 0.09)μg/L respectively]was much higher than that in the model group[(5.516±0.119)pg/ml and(0.76±0.22)μg/L respectively](P＜0.05).The protein expression and serum bone gla protein level was close to that in the sham operation group respectively[(15.279±0.211)pg/ml and(1.34±0.17)μg/L](P＞0.05).Conclusion In the treatment of rats with osteoporosis,Total Flavonoids of Rhizoma Drynaria can obtain a great effect.The possible mechanism is through up-regulation of BMP-2 protein in the femoral bone tissue and the level of serum bone gla protein,thus promoting the formation of bone tissue.

Key words： Osteoporosis Total Flavonoids of Rhizoma Drynaria Bone morphogenetic protein-2 Bone gla protein

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HUANG Song
	CHEN Jing-you
	GAO Hao

Cite this article:

HUANG Song,CHEN Jing-you,GAO Hao. Effect of Total Flavonoids of Rhizoma Drynariae on BMP-2 protein expression and serum bone gla protein level in rats with osteoporosis[J]. 中国当代医药, 2017, 24(4): 12-15.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I4/12

[1]	刘政，吴倩，黄帅立，等.骨质疏松症的中医病因病机与治则研究[J].江苏中医药，2014，46（2）：42-44.
[2]	Wang C，Wang J，Li J，et al.KDM5A controls bone morphogenic protein 2-induced osteogenic differentiation of bone mesenchymal stem cells during osteoporosis[J].Cell Death Dis，2016，7（8）：e2335.
[3]	张春，魏琴，王雪梅，等.BMP-2体外诱导OPG-/-小鼠BMSCs向成骨方向分化的实验研究[J].实验动物与比较医学，2012，32（3）：186-190.
[4]	蔡晓燕，董光富.脂代谢及血清骨钙素水平与骨质疏松症的相关性分析[J].中国骨质疏松杂志，2016，22（6）：711-712，730.
[5]	Ducy P.The role of osteocalcin in the endocrine cross-talk between bone remodelling and energy metabolism[J].Diabetologia，2011，54（6）：1291-1297.
[6]	王春玉，龙丰云，陈香，等.骨钙素介导的骨内分泌系统[J].中华骨质疏松和骨矿盐疾病杂志，2013，6（3）：196-202.
[7]	裴育.骨质疏松症治疗药物研究新进展[J].中国医学前沿杂志（电子版），2015，7（10）：15-20.
[8]	牛爱春，吴建民，郭宪章.中医药治疗骨质疏松症研究现状[J].亚太传统医药，2016，12（9）：47-49.
[9]	曾年，王月义，裘邯军.骨碎补总黄酮对老年骨质疏松症骨痛和骨密度的影响[J].山东中医杂志，2013，32（6）：387-388.
[10]	谢雁鸣，秦林林，邓文龙，等.骨碎补总黄酮对成骨细胞体外培养作用的机制研究[J].中华中医药杂志，2005，20（3）：161-162.
[11]	Huang XF，Yuan SJ，Yang C.Effects of total flavonoids from Drynariafortuneiontheproliferationandosteogenicdifferentiation of rat dental pulp stem cells[J].Mol Med Rep，2012，6（3）：547-552.
[12]	Shibasaki S，Kitano S，Karasaki M，et al.Blocking c-Met signaling enhances bone morphogenetic protein-2-induced osteoblast differentiation[J].FEBS Open Bio，2015，5：341-347.
[13]	王霖霞，李玉坤.BMP-2信号通路与成骨细胞分化[J].国际骨科学杂志，2009，30（2）：132-133.
[14]	Qu X，Cao Y，Chen C，et al.A poly（lactide-co-glycolide）film loaded with abundant bone morphogenetic protein-2：a substrate-promoting osteoblast attachment，proliferation，and differentiation in bone tissue engineering[J].J Biomed Mater Res A，2015，103（8）：2786-2796.
[15]	Price PA，Parthemore JG，Deftos LJ.New biochemical marker for bone metabolism.Measurement by radioimmunoassay of bone GLA protein in the plasma of normal subjects and patients with bone disease[J].J Clin Invest，1980，66（5）：878-883.
[16]	杨伟民，邵斌.骨代谢生化指标与骨质疏松症[J].中国骨质疏松杂志，2004，10（1）：118-121.
[17]	孔德策，杨铁毅，邵进.绝经后骨质疏松骨代谢标志物研究进展[J].国际骨科学杂志，2016，37（1）：36-41.
[18]	潘启源，王直兵，张峡.雌激素通过抑制破骨细胞促进骨软骨缺损的自发修复[J].中华创伤杂志，2016，32（6）：36-39.
[19]	郭军鹏，孟超，于兰英，等.补肾经验方对去卵巢所致大鼠骨质疏松的防治作用[J].吉林中医药，2016，36（5）：65-67.
[20]	惠红岩，董玉珍.自拟补肾活血颗粒对去卵巢骨质疏松症大鼠的治疗作用[J].中国病理生理杂志，2016，（9）：85-88.