p38MAPK信号通路调控IL-6、HIF-1α及VEGF对慢性鼻-鼻窦炎发病机制的影响

Abstract
Figure/Table
References (21)
Related Citation (4)

Download: PDF (0 KB) HTML (49 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To observe the difference of the degree of expression of p38 mitogen-activated protein kinase (p38 MAPK)in chronic rhinosinusitis(CRS)without nasal polyps(CRSsNP),CRS with nasal polyps (CRSwNP)and normal uncinate process,and the expression of interleukin-6 (IL-6),hypoxia-inducible factor 1 alpha (HIF-1α)and vascular endothelial growth factors(VEGF)in different types of CRS.And explore the possible mechanism of p38MAPK signaling pathway in CRS.Methods Patients with nasal endoscopic surgery in department of otolaryngology of Qingdao Municipal Hospital were selected from July 2015 to July 2016 in Qingdao municipal hospital otolaryngology.32 cases of patients with CRSsNP sinus tissue,30 cases of CRSwNP patients with nasal polyps and 24 cases of with normal uncinate mucosa were selected for the study.The protein expression of p38 MAPK in three groups was detected by Western blotting.And the mRNA expression levels of IL-6,HIF-1α and VEGF in three groups were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results The expression of p38MAPK in CRSsNP and CRSwNP was higher than that in normal mucosa,but there was no sig nificant difference between the twogroups(P=0.143).The expression of IL-6 in CRSsNP and CRSwNP was higher than that in normal mucosa,but there was no significant difference between two groups (P=0.082).The expression of HIF-1α and VEGF in CRSwNP was significantly higher than that in normal tissues (P<0.05).While there was no significant difference in the expression of VEGF in CRSsNP and normal tissues(P=0.075).Conclusion p38 MAPK in CRSsNP and CRSwNP shows a different correlation with inflammatory factor IL-6,and the advantage of the expression of IL-6 in CRSsNP and CRSwNP is different,and the expression of IL-6 in CRSsNP is more significant and more closely related.HIF-1a and VEGF are also highly expressed in CRSwNP tissues,which may be related to the production of polyps.

Key words： p38 mitogen-activated protein kinase Interleukin-6 Hypoxia-inducible factor 1 alpha Vascular endothelial growth factor Chronic rhinosinusitis

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	FAN Jun
	ZHAO Chang-min
	LIU Zhao-fang
	HU Wen-ting
	PANG Ming-jie

Cite this article:

FAN Jun,ZHAO Chang-min,LIU Zhao-fang, et al. The influence of p38MAPK signal pathway regulateing expression of IL-6,HIF-1α and VEGF on the chronic rhinosinusitis[J]. 中国当代医药, 2017, 24(9): 8-11转22.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I9/8

[2]	李良波，谭君武，龚成，等.难治性慢性鼻-鼻窦炎的综合治疗[J].临床耳鼻咽喉头颈外科杂志，2015，29（9）：797-799.
[3]	中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组，中华医学会耳鼻咽喉头颈外科学分会鼻科学组.慢性鼻-鼻窦炎诊断和治疗指南（2012年，昆明）[J].中华耳鼻咽喉头颈外科杂志，2013，48（2）：92.
[1]	Wang X，Zhang N，Bo M，et al.Diversity of TH cytokine profiles in patients with chronic rhinosinusitis：a multicenter study in Europe，Asia，and Oceania[J].Allergy Clin Immunol，2016，138（5）：1344-1353.
[4]	Ou M，Sun X，Liang J，et al.A polysaccharide from Sargassum thunbergii inhibits angiogenesis via downregulating MMP-2 activity and VEGF/HIF-1α signaling[J].Int J Biol Macromol，2017，94（Pt A）：451-458.
[5]	刘楠，刘达，刘领波，等.白细胞介素17与血管内皮生长因子在鼻息肉组织中的表达及相关性分析[J].中国耳鼻咽喉头颈外科，2015，22（10）：523-525.
[6]	Shibuya M.Vascular endothelial growth factor（VEGF）and its receptor （VEGFR）signaling in angiogenesis：a crucial target for anti-and pro-angiogenic therapies[J].Cenes Cancer，2011，2（12）：1097-1105.
[7]	刘兆芳，范洪江，逄明杰.p38MAPK与变应性鼻炎的研究进展[J].临床耳鼻咽喉头颈外科杂志，2015，29（23）；2094-2098.
[8]	赵青，万毅刚，王朝俊.慢性肾脏病肾组织炎症信号通路p38MAPK的调节机制及中药的干预作用[J].中国中药杂志，2012，37（12）；1700-1704.
[9]	曲玲，张月琴，陈静.肿瘤坏死因子α，环氧合酶2与p38丝裂原活化蛋白激酶及其信号通路在鼻息肉发病中的作用[J].中国眼耳鼻喉科杂志，2012，12（1）：47-50.
[10]	李舟蕾，任艳鑫，李晓江.鼻咽癌与免疫逃逸[J].国际肿瘤学杂志，2016，43（5）：372-375.
[11]	Scheller J，Garbers C，Rose-John S.Interleukin-6：from basic biology to selective blockade of pro-inflammatory activities[J].Semin Immunol，2014，26（1）：2-12.
[12]	郝志楠，郑勇斌，肖高春，等.丝裂原活化蛋白激酶/细胞外调节蛋白激酶信号通路与肿瘤血管新生的关系[J].中国医药导报，2015，12（1）：148-151.
[13]	张燕，宋冬梅，崔卫娜，等.核因子-κBp65、缺氧诱导因子-1α及血管内皮生长因子在变应性鼻炎小鼠鼻黏膜中的表达[J].中国耳鼻咽喉头颈外科，2013，20（2）：70-73.
[14]	Pereira ER，Frudd K，Awad W，et al.Endoplasmic reticulum（ER）stress and hypoxia response pathways interact to potentiate hypoxia-inducible factor 1（HIF-1）transcriptional activity on targets like vascular endothelial growth factor（VEGF）[J].J Biol Chem，2014，289（6）：3352-3364.
[15]	陈冠男，余飞，盛冠男，等.肿瘤血管生成拟态研究的希望与挑战[J].现代肿瘤医学，2017，25（1）：138-141.
[16]	Geis T，Döring C，Popp R，et al.HIF-2alpha-dependent PAI-1 induction contributes to angiogenesis in hepatocellular carcinoma[J].Exp Cell Res，2015，331（1）：46-57.
[17]	陈冬茹，吴莉萍.低氧诱导因子-1α和反义低氧诱导因子-1α的研究进展[J].国际口腔医学杂志，2016，43（5）：589-593.
[18]	Jing Y，Liu LZ，Jiang Y，et al.Cadmium increases HIF-1 and VEGF expression through ROS，ERK，and AKT signaling pathways and induces malignant transformation of human bronchial epithelial cells[J].Toxicol Sci，2012，125（1）：10-19.
[19]	康建军.支气管哮喘患者血清IL-13、TGF-β-1、VEGF表达变化及临床意义[J].中国医药导报，2015，12（16）：112-114，123.
[20]	肖志超.IL-17、IL-8和IL-6在慢性鼻鼻窦炎中的表达及其意义[D].长沙：中南大学，2013.
[21]	黄正华，陈峰，俞晨杰，等.水通道蛋白5与低氧反应通路因子在鼻息肉组织中的表达及其相关性研究[J].临床耳鼻咽喉头颈外科杂志，2010，24（10）：458-461.