吡格列酮、高糖对3T3-L1细胞中p-Perilipin 1A蛋白表达的影响

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Abstract ObjectiveTo investigate the effects of pioglitazone and high glucose on the expression of p-Perilipin 1A protein in 3T3-L1 cells.Methods3T3-L1 preadipocytes were induced and differentiated into 3T3-L1 adipocytes and then were classified into three groups：control group(5 mmol/L glucose),high glucose group(25 mmol/L glucose)and high glucose plus Pioglitazone group (25 mmol/L glucose+100 μmol/L Pioglitazone).The glycerol content of culture medium was determined and taken as the evaluation index of lipolysis;at the same time,Western blot was used to detect the expression of p-Perilipin 1A protein.ResultsAccording to the release amount of glycerol monitored by using the glycerol kit,a more obvious increase was seen in high glucose group than in control group,with statistically significant difference(P<0.01);while,a more obvious decrease of the release amount of glycerol occurred in high glucose plus Pioglitazone group than that in high glucose group,with statistically significant difference (P<0.01);at the same time,the p-Perilipin 1A proteinof high glucose group detected by Western blot was significantly increased in comparison with control group, with statistically significant difference(P<0.01);and the expression of p-Perilipin 1A protein was significantly inhibited in high glucose group in contrast with high glucose plus pioglitazone group,with statistically significant difference (P< 0.01).ConclusionPioglitazone may prevent the up-regulation of p-Perilipin 1A to inhibit glucose-stimulated lipolysis and decrease free fatty acids,thereby improving the insulin resistance.

Key words： Pioglitazone High glucose 3T3-L1 cells p-Perilipin 1A

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	Articles by authors
	XIA Wen-yan
	XU Rong
	FANG Xiang-nan
	QIN Yong-zhang
	OUYANG Long-qiang

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XIA Wen-yan,XU Rong,FANG Xiang-nan, et al. Effect of Pioglitazone and high glucose on the expression of p-Perilipin 1A protein in 3T3-L1 cells[J]. 中国当代医药, 2017, 24(12): 9-11转19.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I12/9

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