电化学发光免疫法和酶联免疫法检测乙肝病毒标志物结果准确性的比较

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Abstract ObjectiveTo analyze and compare the accuracy of electrochemiluminescence immunoassay(ECLIA)and enzyme-linked immunosorbent assay(ELISA)in the detection of hepatitis B virus(HBV)markers.Methods160 patients with HBV treated in our hospital from January 2015 and January 2017 were selected as research targets,and were randomly divided into the observation group(n=80)and the control group(n=80).The observation group was given ECLIA and the control group was given ELISA,the positive rates of HBV markers were compared between the two groups.Then in the observation group,HBsAg/Ab and/or HBeAg/Ab double positive patients were selected and detected by ELISA method,and the results were observed.ResultsThe positive rates of HBsAg,HBsAb,HBeAg and HBeAb in the observation group were 100.0%,52.5%,76.3%and 78.8%respectively,which was higher than 92.5%,45.2%,52.5%,50.0%in the control group,with significant difference(P＜0.05).The number of patients with the s and/or e system double positive detection results by the method of ECLIA were 76 cases,but the number by the method of ELISA were comparatively 9 cases.ConclusionCompared with ELISA,ECLIA is more accurate in the detection of HBV infection,therefore,it is worthy of wide clinical promotion and application.

Key words： Electrochemiluminescence immunoassay Enzyme-linked immunosorbent assay Hepatitis B virus

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	SHI Zhen-gang

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SHI Zhen-gang. Comparison of the accuracy of electrochemiluminescence immunoassay and enzyme-linked immunosorbent assay in the detection of hepatitis B virus markers[J]. 中国当代医药, 2017, 24(18): 111-113.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I18/111

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