辛伐他汀对高胆固醇血症大鼠APP、BACE蛋白表达的影响

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Abstract ObjectiveTo explore the efficacy of Simvastatin on the expression of APP and BACE in rats with hypercholesterolemia,providing theoretical basis for the prevention and treatment in Alzheimer′s Disease.MethodsThe 32 rats used in this experiment were 3 months old and randomly divided into normal diet group(n=16)and high cholesterol diet groups(n=16).The high diet groups were randomly divided into high lipid feed the placebo group(n=8)and high-fat feed drug group(n=8).And then medicine was filled into their stomach:the high fat diet drugs group were given simvastatin 2 mg/（kg·d）,the high fat diet placebo group placebo(distilled water)by the same volume,the high fat forage during lavage.Normal feed group were randomly divided into normal feed placebo group and normal feed drug group,respectively to the same volume with the placebo(distilled water)and simvastatin 2 mg/（kg·d）to fill the stomach,the normal fat forage during lavage,with behavior evaluation after 8 weeks.Immune histochemical method to determine the brain′s protein expression with APP,BACE.ResultsThe result of APP,BACE protein expression in the prefrontal cortex and the hippocampus with immunohistochemical detection:the protein expression of APP and BACE in the prefrontal cortexis and the hippocampus was decreased in high fat feed drug group than the normal feed placebo group(P＜0.05),there was no significant difference in the normal feed placebo group,the normal feed drug group and the high fat feed drug group (P＞0.05).ConclusionSimvastatin may affect the protein expression of APP,BACE in the prefrontal cortex and the hippocampus by the hypercholesterolemia rats,which have an impact on the metabolism of Aβ1-42.

Key words： Simvastatin Hypercholesterolemia The hippocampus Cortex APP BACE Alzheimer&prime s Disease

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