虫草素对高糖介导大鼠肾小管上皮细胞p38MAPK通路及TGF-β1表达的影响

Abstract
Figure/Table
References (20)
Related Citation (15)

Download: PDF (0 KB) HTML (48 KB)
Export: BibTeX | EndNote (RIS)

Abstract ObjectiveTo investigate effect of cordycepin on p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose.MethodsNRK52E cells were cultured in the mediun with normal glucose concentration（group NG）,high glucose concentration（group HG）and high glucose concentration+cordycepin（group HG+C）.The expression of p38MAPK and TGF-β1mRNA was measured by quantitative RT-PCR;the expression level of p-p38MAPK and TGF-β1protein was detected by Western blot.ResultsThe p38MAPK and TGF-β1mRNA in NRK52E cells were highly expressed in group HGcompared with group NG（P＜0.01）;the tendency of p-p38MAPK and TGF-β1protein expressed was accordance with expression of mRNA.However,cordycepin can significantly inhibit the activation of p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose.ConclusionCordycepin can significantly inhibit the activation of p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose.

Key words： P38MAPK Cordycepin TGF-&beta 1 Glucose

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LIANG Ze-zhi
	HUANG Jie-ping

Cite this article:

LIANG Ze-zhi,HUANG Jie-ping. Effect of cordycepin on p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose[J]. 中国当代医药, 2017, 24(17): 4-7.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I17/4

[1]	Nazir N，Siddiqui K，Al-Qasim S，et al.Meta-analysis of diabeticnephropathyassociatedgeneticvariantsin inflammation and angiogenesis involved in different biochemical pathways[J].BMC Med Genet，2014，15（1）：103.
[2]	Sasai Y，Iwakawa K，Yanagida K，et al.Advanced glycation endproducts stimulate renal epithelial cells to release chemokines that recruit macrophages，leading to renal fibrosis[J]. Biosci Biotechnol Biochem，2012，76（9）：1741-1745.
[3]	Goldberg R，Rubinstein AM，Gil N，et al.Role of heparanasedriven inflammatory cascade in pathogenesis of diabetic nephropathy[J].Diabetes，2014，63（12）：4302-4313.
[4]	Marketou NP，Chrousos GP，Kanaka-Gantenbein C.Diabetic nephropathy in type 1 diabetes：a review of early natural history，pathogenesis and diagnosis[J].Diabetes Metab Res Rev，2016.
[5]	Rane MJ，Song Y，Jin S，et al.Interplay between Akt and p38 MAPK pathways in the regulation of renal tubular cell apoptosis associated with diabetic nephropathy[J].Am J Physiol Renal Physiol，2010，298（1）：F49-F61.
[6]	Li X，Liu W，Wang Q，et al.Emodin suppresses cell proliferation and fibronectin expression via p38MAPK pathway in rat mesangial cells cultured under high glucose[J].Mol Cell Endocrinol，2009，307（1）：157-162.
[7]	Xu Q，Tan Y，Zhang K，et al.Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells[J]. Int J Oncol，2013，43（4）：1187-1193.
[8]	Kanasaki K，Taduri G，Koya D.Diabetic nephropathy：the role of inflammation in fibroblast activation and kidney fibrosis[J].Front Endocrinol，2013，4（1）：7.
[9]	Chung ACK，Lan HY.Molecular mechanisms of TGF-β signaling in renal fibrosis[J].Curr Pathobiol Rep，2013，1（4）：291-299.
[10]	Zeisberg M，Hanai J，Sugimoto H，et al.BMP-7 counteracts TGF-β1-induced epithelial-to-mesenchymal transition and reverses chronic renal injury[J].Nat Med，2003，9（7）：964-968.
[11]	Lan HY.Diverse roles of TGF-beta/Smads in renal fibrosis and inflammation[J].Int J Biol Sci，2011，7（7）：1056-1067.
[12]	Meng XM，Chung ACK，Lan HY.Role of the TGF-β/BMP-7/ Smad pathways in renal diseases[J].Clin Sci（Lond），2013，124（4）：243-254.
[13]	Lv ZM，Wang Q，Wan Q，et al.The role of the p38 MAPK signaling pathway in high glucose-induced epithelial-mesenchymal transition of cultured human renal tubular epithelial cells[J].PLoS One，2011，6（7）：e22806.
[14]	Lan HY.Transforming growth factor-β/Smad signalling in diabetic nephropathy[J].Clin Exp Pharmacol Physiol，2012，39（8）：731-738.
[15]	Rhyu DY，Yang Y，Ha H，et al.Role of reactive oxygen species in TGF-β1-induced mitogen-activated protein kinase activation and epithelial-mesenchymal transition in renal tubular epithelial cells[J].J Am Soc Nephrol，2005，16（3）：667-675.
[16]	Tzeng TF，Liou SS，Chang CJ，et al.The ethanol extract of Lonicera japonica（Japanese honeysuckle）attenuates diabetic nephropathy by inhibiting p-38 MAPK activity in streptozotocin-induced diabetic rats[J].Planta Med，2014，80（2/3）：121-129.
[17]	周巧玲，刘抗寒，王衍慧，等.冬虫夏草对糖尿病肾病模型鼠肾组织转化生长因子B1，结缔组织生长因子表达的影响[J].肾脏病与透析肾移植杂志，2006，15（5）：443-446.
[18]	叶太生，周必发，张莹雯.百令胶囊对大鼠肾小球系膜细胞增殖，Ⅳ型胶原及TGF-β1mRNA表达的影响[J].中国中西医结合肾病杂志，2010，11（1）：41-43.
[19]	Baik JS，Kwon HY，Kim KS.Cordycepin induces apoptosis inhumanneuroblastomaSK-N-BE（2）-CandmelanomaSKMEL-2 cells[J].Indian J Biochem Biophys，2012，49（2）：86-91.
[20]	Cui J，Culbertson R，Mao Z，et al.A novel therapeutic treatment utilizing cordycepin and cladribine synergy to decrease adverse treatment effects in various cancer cell lines[J]. JESS，2013，2：20-24.