不同氧浓度进行培养液的平衡对辅助生殖临床结局的影响

Abstract
Figure/Table
References (22)
Related Citation (0)

Download: PDF (605 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective This study aims to investigate the impact of different oxygen concentrations on the clinical outcomes of assisted reproduction by balancing culture media.Methods Patients' data on assisted reproduction in the Center of Reproductive Medicine of Fujian Maternity and Child Health Hospital from March 2020 to September 2021 was retrospectively analyzed,with a total of 401 egg retrieval cycles meeting the inclusion criteria.They were divided into atmospheric oxygen balance group and 5% oxygen balance group based on the use of different oxygen concentration incubators.Among them,the atmospheric oxygen balance group had 201 cycles and the 5%oxygen balance group had 200 cycles.The in vitro embryonic development and clinical outcomes of patients of the two groups were compared and analyzed.Results There was no statistically significant difference in baseline data between the two groups of patients (P＞0.05).The normal fertilization rate and high-quality blastocyst formation rate of the atmospheric oxygen balance group were higher than those of the 5%oxygen balance group,and the differences were statistically significant(P＜0.05).There were no statistically significant differences between the two groups of patients in terms of multiple fertilization rate,D3 high-quality embryo rate,blastocyst formation rate,clinical pregnancy rate,implantation rate,abortion rate,neonatal pregnancy time,neonatal birth weight and neonatal birth length (P＞0.05).Conclusion During the routine cycles of in vitro fertilization and embryo transfer,using a regular incubator with atmospheric oxygen concentration to balance the culture medium may be beneficial for normal fertilization and high-quality blastocyst formation in patients.

Key words： Oxygen concentration Balance culture media Incubator Fertilization rate Blastocyst formation rate

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

https://www.dangdaiyiyao.com/EN/ OR https://www.dangdaiyiyao.com/EN/Y2023/V30/I21/76

[1]	Niederberger C，Pellicer A，Cohen J，et al.Forty years of IVF[J].Fertil Steril，2018，110（2）：185-324.
[2]	Gardner DK，Sakkas D.Making and selecting the best embryo in the laboratory[J].Fertil Steril，2022，118（6）：1170-1179.
[3]	Gelo N，Kirinec G，Baldani DP，et al.Influence of human embryo cultivation in a classic CO2incubator with 20% oxygen versus benchtop incubator with 5% oxygen on live births：the randomized prospective trial[J].Zygote，2019，27（3）：131-136.
[4]	Kirkegaard K，Hindkjaer JJ，Ingerslev HJ.Effect of oxygen concentration on human embryo development evaluated by time-lapse monitoring[J].Fertil Steril，2013，99（3）：738-744.
[5]	Swain JE.Media composition：pH and buffers[J].Methods Mol Biol，2012，912：161-175.
[6]	徐慧灵，郑备红，邱淑敏，等.多囊卵巢综合征患者体外受精控制性超排卵方案的选择[J].海峡预防医学杂志，2017，23（4）：94-96.
[7]	Cooper TG，Noonan E，von Eckardstein S，et al.World Health Organization reference values for human semen characteristics[J].Hum Reprod Update，2010，16（3）：231-245.
[8]	ESHREClinicPIWorkingGroup，VlaisavljevicV，ApterS，et al.The Maribor consensus：report of an expert meeting on the development of performance indicators for clinical practice in ART[J].Hum Reprod Open，2021，2021（3）：hoab022.
[9]	Gardner DK，Lane M，Stevens J，et al.Blastocyst score affects implantation and pregnancy outcome：towards a single blastocyst transfer[J].Fertil Steril，2000，73（6）：1155-1158.
[10]	Lin Y，Da L，Du S，et al.The vitrification system may affect preterm and cesarean delivery rates after single vitrified blastocyst transfer[J].Syst Biol Reprod Med，2022，68（2）：113-120.
[11]	陶林林，李国臻，杨志伟，等.辅助生殖技术中培养液低氧平衡对胚胎早期发育及妊娠结局的影响[J].中国计划生育和妇产科，2020，12（7）：57-61.
[12]	陶林林，李国臻，杨志伟，等.培养液提前一日低氧平衡和高氧平衡对不同授精方式胚胎早期发育及妊娠结局的影响[J].中华生殖与避孕杂志，2022，42（12）：1234-1240.
[13]	Kleijkers SH，van Montfoort AP，Smits LJ，et al.IVF culture medium affects post-natal weight in humans during the first 2 years of life[J].Hum Reprod，2014，29（4）：661-669.
[14]	Tao P，Zhou W，Yan X，et al.Effect of sequential versus single-step culture medium on IVF treatments，including embryo and clinical outcomes：a prospective randomized study[J].Arch Gynecol Obstet，2022，305（3）：757-765.
[15]	Zheng Y，Dong X，Sui C，et al.Culture medium is associated with the risks of placenta previa and macrosomia in pregnancies after in vitro fertilization[J].Arch Gynecol Obstet，2022，306（1）：239-247.
[16]	杨弘睚，媛马，石永乾，等.不同受精方式对早发性卵巢功能不全患者胚胎质量和妊娠结局的影响[J].中国妇幼健康研究，2021，32（9）：1259-1264.
[17]	陈鑫，徐鸿毅，张鑫，等.获卵数及可移植囊胚数与PGT-M/PGT-SR 助孕周期累积活产率的相关性研究[J].生殖医学杂志，2022，31（4）：468-475.
[18]	郭岩文，郑娟，蔡嘉力，等.早卵泡期长方案与拮抗剂方案治疗卵巢储备功能下降年轻患者的效果比较[J].中国当代医药，2019，26（15）：62-64.
[19]	Christianson MS，Zhao Y，Shoham G，et al.Embryo catheter loading and embryo culture techniques：results of a worldwide Web-based survey[J].J Assist Reprod Genet，2014，31（8）：1029-1036.
[20]	Glatthorn HN，Hanson BM，Kim JG，et al.Individual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single，vitrifiedwarmed euploid blastocyst transfer[J].J Assist Reprod Genet，2021，38（8）：2157-2164.
[21]	Wale PL，Gardner DK.Time-lapse analysis of mouse embryo development in oxygen gradients[J].Reprod Biomed Online，2010，21（3）：402-410.
[22]	Herbemont C，Labrosse J，Bennani-Smires B，et al.Impact of oxygen tension according to embryo stage of development：a prospective randomized study[J].Sci Rep，2021，11（1）：22313.