Objective To study the clinical effect of Phloroglucinol and Etimicin in the treatment of patients with spasmodic abdominal pain caused by acute gastroenteritis (AG). Methods A total of 104 patients with spasmodic abdominal pain caused by AG treated in the Department of Emergency, the First People's Hospital of Jiujiang City from October 1, 2021 to March 15, 2022 were selected as the research subjects, and they were divided into control group (n=52) and observation group (n=52) according to random number table method.The control group was injected with Racemic Anisodamine Hydrochloride and Levofloxacin Hydrochloride and Sodium Chloride, and the observation group was injected with Phloroglucinol and Etimicin.The levels of inflammatory cytokines (interleukin-6 [IL-6]and interleukin-8[IL-8]) between the two groups before and after 3 days of treatment were compared.The duration of symptoms (diarrhea, hyperthermia, nausea and vomiting, abdominal pain) of the two groups were compared.The clinical efficacy of the two groups was compared. Results After 3 days of treatment, the levels of IL-6 and IL-8 in the observation group were lower than those in the control group, the differences were statistically significant (P＜0.05).The duration of diarrhea,hyperthermia, nausea and vomiting, and abdominal pain in the observation group were shorter than those in the control group, the differences were statistically significant (P＜0.05).The total effective rate of the observation group was higher than that of the control group, the difference was statistically significant (P＜0.05). Conclusion Phloroglucinol and Etimicin in the treatment of patients with spasmodic abdominal pain caused by AG can effectively reduce the inflammatory response of patients, rapidly relieve clinical symptoms, and have good clinical effect.