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| Content determination of AE from the leaves of Actinidia eriantha Benth.and study on the extraction and purification process |
| WANG Yong-hong1 WANG Xue-ying2 PENG Shi-lu2 YU Qi-nan2 LI Hong-liang2▲ |
1. Taiji Group Southwest Pharmaceutical Co., LTD., Chongqing 400000, China;
2. Taiji Group Chongqingfulin Pharmaceutical Co., LTD., Chongqing 408000, China |
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Abstract Objective To establish the method for determination of 2α,3α,24-trihydroxyurs-12-en-28-oic acid (AE)from the leaves of Actinidia eriantha Benth., and optimize the extraction and purification process. Methods The content determination method of AE was established by high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) using AE as reference substance. The optimal extraction process was researched by single factor test and orthogonal test. The optimal purification process was researched by single factor test. Results The stable content determination method of AE was established, and it was found that the content of AE in Actinidia eriantha Benth. leaves from 5 different habitats was quite different. The optimal process of the extraction was as follows: extraction for 2 hours with 15-fold 70% ethanol in the first time, and extraction for 1 hour with 10-fold 70% ethanol in the second time. The optimal purification process was as follows: concentrating the extract to 0.50 g crude drug/ml, discarding the supernatant after sit for 8 hours, adding 30% ethanol into the sediment to let the concentration become 0.50 g crude drug/ml, and stirring thoroughly, discarding the supernatant after sit for 8 hours again, adding the appropriate amount of petroleum ether (60-90) into the sediment, and washing thoroughly. The average content of AE intermediate was 36.25%, and the yield from medicinal materials to intermediates also reached 55.96%. Conclusion The method established in this experiment is stable and feasible, and can be used for the determination of AE in Actinidia eriantha Benth. leaves, the optimized process is simple, stable and repeatable, which is suitable for industrial production.
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| [1] |
江苏新医学院.中药大辞典[M].上海：上海科学技术出版社，1977，440.
|
| [2] |
福建省医药研究所.福建中草药（第一册）[M].福建：福建省新华书店出版社，1970，575.
|
| [3] |
浙江省革命委员会生产指挥组卫生局.浙江民间常用草药[M].杭州：浙江人民出版社，1970，54.
|
| [4] |
郭辉辉.毛花猕猴桃氯仿层单体化合物分离及其抗肿瘤作用机制研究[D].杭州：浙江理工大学，2013.
|
| [5] |
黄初升，张壮鑫，李干孙，等.毛花猕猴桃中的两个新三萜化合物[J].云南植物研究，1988，10（1）：93-100.
|
| [6] |
黄初升，陈希慧.毛花猕猴桃根中的一个新三萜化合物[J].天然产物研究与开发，1992，4（3）：27-30.
|
| [7] |
白素平，黄初升，陈希慧.毛花猕猴桃三萜化学成分的研究[J].天然产物研究与开发，1997，9（1）：15-18.
|
| [8] |
郑燕枝，李仁强，吴建国，等.毛花猕猴桃总黄酮的超声提取工艺[J].福建中医药大学学报，2012，22（3）：50-51.
|
| [9] |
Xu HS，Wu YW，Xu SF，et al.Antitumor and immunomodulatory activity of polysaccharides from the roots of Actinidia eriantha[J].J Ethnopharmacol，2009，125（2）：310-317.
|
| [10] |
林水花，吴建国，谢通，等.毛花猕猴桃不同部位的抗肿瘤活性比较[J].福建中医药大学学报，2013，23（1）：46-47.
|
| [11] |
张家敏，张骋，王鹏.白藤梨根体外抗肿瘤作用部位筛选及总三萜含量测定[J].中国现代应用药学，2019，36（10）：1232-1235.
|
| [12] |
Zhang D，Gao C，Li R，et al.TEOA，a triterpenoid from Actinidia eriantha，induces autophagy in SW620 cells via endoplasmic reticulum stress and ROS-dependent mitophagy[J].Arch Pharm Res，2017，40（5）：579-591.
|
| [13] |
Wu JG，Ma L，Lin SH，et al.Anticancer and anti-angiogenic activities of extract from Actinidia eriantha Benth root[J].J Ethnopharmacol，2017，203：1-10.
|
| [14] |
林水花，吴锦忠，吴建国.毛花猕猴桃根中总三萜成分纯化工艺研究[J].海峡药学，2017，29（5）：29-32.
|
| [15] |
易骏，吴建国，郑燕枝，等.正交设计法优化毛花猕猴桃根三萜超声提取工艺[J].海峡药学，2018，30（3）：6-8.
|
| [16] |
卢泳庄.毛花猕猴桃根活性部位与活性成分制备及其抗肿瘤活性研究[D].杭州：浙江大学，2015.
|
| [17] |
宫明华，李瑶函，薛玲，等.HPLC 测定毛花猕猴桃根中活性成分2α，3α，24-三羟基-12-烯-28-乌苏酸的含量[J].中国药学杂志，2016，51（14）：1219-1223.
|
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