MTHFR C677T基因多态性与同型半胱氨酸及冠心病的关系

Abstract
Figure/Table
References (21)
Related Citation (15)

Download: PDF (0 KB) HTML (38 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the relationship between C677T polymorphism of methylenetetrahydrofolate reductase (MTHFR) and the level of plasma homocysteine (Hcy) as well as coronary heart disease (CHD). Methods From December 2016 to October 2019, 153 patients with CHD from Fuxin Fifth People′s Hospital and the First Affiliated Hospital of Jinzhou Medical University were selected as CHD group, and 149 subjects for healthy examination were selected as the control group at the same period. The clinical data of biochemical indexes such as blood lipid and Hcy were collected. The plasma Hcy level was measured by enzyme circulation method. The blood samples were taken to extract genomic DNA, and the C677T polymorphism of MTHFR gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The Hcy level was the lowest in CC genotype and the highest in TT genotype in CHD group (P＜0.05). In the control group, the Hcy level in different genotypes showed no significant difference (P＞0.05). The proportions of CC genotype and C allele frequency in the CHD group were lower than those in the control group, and the differences were statistically significant (P＜0.05). The proportions of TT genotype and T allele frequency in the CHD group were higher than those in the control group, and the differences were statistically significant (P＜0.05). TT genotype was an independent risk factor for CHD (OR=4.388, 95%CI: 2.091-5.695, P=0.001), and CC genotype was an independent protective factor for CHD (OR=0.590, 95%CI: 0.393-0.921, P=0.001). Conclusion TT genotype in MTHFR C677T polymorphism is a risk factor for CHD. The plasma Hcy level is closely related to it, the increase of plasma Hcy level is related to TT genotype mutation and T allele. Hcy level in plasma and MTHFR C677T polymorphism may be an important reference indexes to study the etiology, pathogenesis and genetic mechanism of CHD.

Key words： Homocysteine Methylenetetrahydrofolate reductase Coronary heart disease Gene polymorphism

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

https://www.dangdaiyiyao.com/EN/ OR https://www.dangdaiyiyao.com/EN/Y2020/V27/I26/4

[1]	Karadeniz M，Sarak T，Duran M，et al.Hyperhonocysteinemia predicts the severity of coronary artery disease as determined by the SYNTAX score in patients with Acute coronary syndrome[J].Acta Cardiol Sin，2018，34（6）：458-463.
[2]	Tang Z，Xiao L，Wang JQ，et al.Analysis of metabolism-related indicators and MTHFR gene polymorphism in patients with H-type hypertension[J].Minerva Med，2017，108（2）：103-107.
[3]	Miwa K，Tanaka M，Okazaki S，et al.Increased Total Homocysteine Levels Predict the Risk of Incident Dementia Independent of Cerebral Small-Vessel Diseases and Vascular Risk Factors[J].J Alzheimers Dis，2016，49（2）：503-513.
[4]	Khera AV，Kathiresan S.Genetics of coronary artery disease：discovery，biology and clinical translation[J].Nat Rev Genet，2017，18（6）：331-344.
[5]	龚艳君，霍勇.急性ST 段抬高型心肌梗死诊断和治疗指南（2019）解读[J].中国心血管病研究，2019，17（12）：1057-1061.
[6]	刘巍.非ST 段抬高急性冠脉综合征的诊断及治疗2014年AHA/ACC NSTE-ACS 指南解读[J].中西医结合心血管病电子杂志，2015，3（3）：1-3，207.
[7]	冯杏琳，申华，罗素霞，等.MTHFR C677T 基因多态性分布与不良妊娠结局的关系[J].中国优生与遗传杂志，2018，23（11）：16-17.
[8]	戴岭，王银瓶，佘广彤，等.血叶酸、同型半胱氨酸及尿酸水平与妊娠期高血压疾病发病的相关性分析[J].中国妇产科临床杂志，2017，18（4）：361-362.
[9]	Zhou BS，Bu GY，Li M，et al.Tagging SNPs in the MTHFR gene and risk of ischemic stroke in a Chinese population[J].Int J Mol Sci，2014，15（5）：8931-8940.
[10]	S rensen JT，Gaustadenes M，Stabler SP，et al.Molecular and biochemical investigations of patients with intermediate or severe hyperhomocysteinemia[J].Mol Genet Metab，2016，117（3）：344-350.
[11]	Li A，Shi Y，Xu L，et al.A possible synergistic effect of MTHFR C677T polymorphism on homocysteine level variations increased risk for ischemic stroke[J].Medicine（Baltimore），2017，96（51）：e9300.
[12]	Liu W，Wang T，Sun P，et al.Expression of Hcy and blood lipid levels in serum of CHD patients and analysis of risk factors for CHD[J].Exp Ther Med，2019，17（3）：1756-1760.
[13]	Zhang Z，Yan Q，Guo J，et al.A plasma proteomics method reveals links between ischemic stroke and MTHFR C677T genotype[J].Sci Rep，2017，7（1）：13 390.
[14]	许少英.血透患者血浆HCY 和叶酸、VitB12 浓度关系分析及干预研究[J].临床医学工程，2020，27（2）：171-172.
[15]	黎国德，许庆波，韩克栋.MTHFR 基因多态性对冠心病合并高血压患者氯吡格雷耐受性的影响[J].广东医学，2020，41（1）：91-96.
[16]	Almawi WY，AmeenG，Tamim H，et al.Factor V G1691A,prothrombin G20210A, and methylenetetrahydrofolate reductase[MTHFR]C677T gene polymorphism in angiographically documented coronary artery disease[J].J Thrombolysis，2014，17（3）：199-205.
[17]	Sarecka-Hujar B，Zak I，Krauze J，et al.The TT genotype of the MTHFR 677C > T polymorphism increases susceptibility to premature coronary artery disease in interaction with some of the traditional risk factors[J].Acta Medica（Hradec Kralove），2012，55（4）：172-179.
[18]	于善花，陈皆春，庄爱霞，等.亚甲基四氢叶酸还原酶基因多态性与分支动脉粥样硬化疾病早期神经恶化相关性研究[J].中华老年心脑血管病杂志，2019，21（12）：1301-1304.
[19]	Li MN，Wang HJ，Zhang NR，et al.MTHFR C677T gene polymorphism and the severity of coronary lesions in acute coronary syndrome[J].Medicine（Baltimore），2017，96（49）：e9044.
[20]	Burdennyy AM，Loginov VI，Zavarykina TM，et al.The role of molecular genetic alterations in genes involved in folate and homocysteine metabolis m in multifactorial diseases pathogenesis[J].Russ J Genet，2017，53（5）：528-541.
[21]	农茜，陆文权，潘兴寿，等.彝族高血压患者MTHFR C677T 基因多态性及其与血压、同型半胱氨酸水平的关系[J].中国医药科学，2019，9（19）：9-13.