Objective To explore the application effect of flipped classroom combined with teaching competition in the training of newly recruited nurses in operating room. Methods Fifteen newly recruited nurses who entered our hospital from June to August 2017 were selected as control group, and 15 newly recruited nurses who entered our hospital from June to August 2018 were selected as observation group.The control group adopted ″tutor mentoring and core competence training″ mode, and in the observation group″ flipped classroom and teaching competition″ was added on the basis of the control group. The time of training for both groups was one year. The assessment results (theory and operation), the satisfaction rate of the newly recruited nurses with the training, and the satisfaction rate of the doctors with the newly recruited nurses in the two groups were recorded. Results The theoretical and operational scores of the observation group were (91.89±2.80) points and (90.10±1.70) points, respectively. The theoretical and operational scores of the control group were (80.74±3.33) points and (83.38±3.09) points, respectively. The theoretical and operational scores of the observation group were higher than those of the control group, and the differences were statistically significant (P＜0.01）. The observation group′s satisfaction with training was higher than that of the control group, and the difference was statistically significant (P＜0.01）. The doctors′ satisfaction of the observation group was higher than that of the control group, and the difference was statistically significant (P＜0.01）. Conclusion The tutor system has been a teaching mode in operating room for a long time, and the core competence training is a standardized training with comprehensive content and strong scientificity. Flipped classroom and teaching competition can greatly increase the enthusiasm of tutors and new nurses. It has the characteristics of inheritance，scientificity and innovation, which is worthy of promoting.