Objective To investigate the effect of whole blood storage temperature and time on quality of cryoprecipitate coagulation factor. Methods A total of 120 bags (200 ml/bag) of whole blood collected in Central Blood Station of Foshan City from January to June in 2020 were selected as research objects and divided into room temperature group and low temperature group with 60 bags in each group. Each group was divided into three parts on average, 20 bags per part, and stored at room temperature (20-24℃) and low temperature (2-6℃) for 8, 12 and 24 h.Fresh frozen plasma was obtained by centrifugation, and cryoprecipitated coagulation factor was prepared within one week. The content of coagulation factor Ⅷ (FⅧ), the content of fibrinogen (FIB), the qualified rate of FⅧand the qualified rate of FIB in cryoprecipitated coagulation factor were determined. The content of FⅧ, the content of FIB, the qualified rate of FⅧand the qualified rate of FIB were compared between the two groups at different storage temperature and time. Results There were no statistically significant differences in the contents of FⅧand FIB stored for 8, 12, 24 h between the two groups (P>0.05). There were no statistically significant differences in the contents of FⅧand FIB stored for 8 h and 12 h (P>0.05). The contents of FⅧand FIB stored for 24 h in the two groups were higher than those stored for 8 h, and the differences were statistically significant (P<0.05). In the low temperature group stored for 8, 12, 24 h, the FⅧqualified rate was 100.00%, 95.00% and 90.00% respectively, compared with room temperature group of 100.00%, 95.00%, 85.00%, there were no statistically significant differences (P>0.05). In the low temperature group stored for 8, 12, 24 h, the qualified rates of FIB were 100.00%, 100.00%, and 95.00%, respectively, which showed no statistically significant differences compared with 100.00%, 100.00%, and 95.00% in the room temperature group (P>0.05). The FIB qualified rates of the two groups stored for 12, 24 h were compared with stored for 8 h, and the differences were not statistically significant (P>0.05). Conclusion There is no significant difference in the activity of cryoprecipitated coagulation factor between whole blood stored at room temperature and at low temperature within 24 h.